› 4jl7

X-ray diffraction
1.16Å resolution

Crystal Structure of the Chemokine Receptor CXCR2 in Complex with the First PDZ Domain of NHERF1

Released:
DOI: 10.2210/pdb4jl7/pdb
PDB entry 4jl7 coloured by chain, front view.
PDB entry 4jl7 coloured by chain, side view.
PDB entry 4jl7 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into neutrophilic migration revealed by the crystal structure of the chemokine receptor CXCR2 in complex with the first PDZ domain of NHERF1.
Lu G, Wu Y, Jiang Y, Wang S, Hou Y, Guan X, Brunzelle J, Sirinupong N, Sheng S, Li C, Yang Z
PLoS One 8 e76219 (2013)
PMID: 24098448

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127084 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Na(+)/H(+) exchange regulatory cofactor NHE-RF1 Chain: A
Molecule details ›
Chain: A
Length: 91 amino acids
Theoretical weight: 9.86 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O14745 (Residues: 11-95; Coverage: 24%)
Gene names: NHERF, NHERF1, SLC9A3R1
Sequence domains: PDZ domain
Structure domains: Pdz3 Domain

Ligands and Environments

1 bound ligand:
Ligand CL 3 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P3121
Unit cell:
a: 50.36Å b: 50.36Å c: 65.982Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.187 0.186 0.208
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Role of the PDZ-scaffold protein NHERF1/EBP50 in cancer biology: from signaling regulation to clinical relevance.
Vaquero et al. (2017)
3 more

3 mentions without citation

Structural insights into neutrophilic migration revealed by the crystal structure of the chemokine receptor CXCR2 in complex with the first PDZ domain of NHERF1.
Lu et al. (2013)
2 more