› 4jl0

X-ray diffraction
2.22Å resolution

Crystal structure of PcrH in complex with the chaperone binding region of PopB

Released:
DOI: 10.2210/pdb4jl0/pdb
PDB entry 4jl0 coloured by chain, front view.
PDB entry 4jl0 coloured by chain, side view.
PDB entry 4jl0 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa CHA
Primary publication:
Membrane and chaperone recognition by the major translocator protein PopB of the type III secretion system of Pseudomonas aeruginosa.
Discola KF, Förster A, Boulay F, Simorre JP, Attree I, Dessen A, Job V
J Biol Chem 289 3591-601 (2014)
PMID: 24297169

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128250 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Chaperone protein IpgC Chains: A, B
Molecule details ›
Chains: A, B
Length: 142 amino acids
Theoretical weight: 15.49 KDa
Source organism: Pseudomonas aeruginosa CHA
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O30528 (Residues: 22-161; Coverage: 83%)
Gene names: GNQ48_01015, IPC737_28835, PAERUG_P19_London_7_VIM_2_05_10_06388, ipgC, pcrH
Structure domains: Tetratricopeptide repeat domain
Translocator protein BipB-like C-terminal domain-containing protein Chains: C, D
Molecule details ›
Chains: C, D
Length: 9 amino acids
Theoretical weight: 842 Da
Source organism: Pseudomonas aeruginosa CHA
Expression system: Not provided
UniProt:
  • Canonical: Q840U9 (Residues: 51-59; Coverage: 2%)
Gene name: popB

Ligands and Environments

1 bound ligand:
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Unit cell:
a: 45.1Å b: 73.29Å c: 98.94Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.214 0.265
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

6 review citations

Type Three Secretion System in Attaching and Effacing Pathogens.
Gaytán et al. (2016)
5 more

3 mentions without citation

Membrane and chaperone recognition by the major translocator protein PopB of the type III secretion system of Pseudomonas aeruginosa.
Discola et al. (2014)
2 more