4jk0

X-ray diffraction
2.3Å resolution

Crystal structure of T89Q-mutant of RNA silencing suppressor p19 with 2nt-5'-overhanging double-helical RNA 21mer pUUUG(CUG)5CU

Released:
Source organism: Tomato bushy stunt virus
Entry authors: Katorcha E, Tamjar J, Popov AN, Malinina L

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
RNA silencing suppressor p19 Chain: D
Molecule details ›
Chain: D
Length: 127 amino acids
Theoretical weight: 14.54 KDa
Source organism: Tomato bushy stunt virus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P69517 (Residues: 27-149; Coverage: 72%)
Gene name: ORF4
Structure domains: RNA silencing suppressor P19
5'-R(P*UP*UP*UP*GP*CP*UP*GP*CP*UP*GP*CP*UP*GP*CP*UP*GP*CP*UP*GP*CP*U)-3' Chain: B
Molecule details ›
Chain: B
Length: 21 nucleotides
Theoretical weight: 6.61 KDa
Source organism: Tomato bushy stunt virus
Expression system: Not provided

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: R32
Unit cell:
a: 90.391Å b: 90.391Å c: 148.909Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.187 0.184 0.246
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided