4je5

X-ray diffraction
1.91Å resolution

Crystal structure of the aromatic aminotransferase Aro8, a putative alpha-aminoadipate aminotransferase in Saccharomyces cerevisiae

Released:

Function and Biology Details

Reactions catalysed:
L-2-aminoadipate + 2-oxoglutarate = 2-oxoadipate + L-glutamate
An aromatic amino acid + 2-oxoglutarate = an aromatic oxo acid + L-glutamate
Biochemical function:
Cellular component:

Structure analysis Details

Assemblies composition:
homo dimer
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Aromatic/aminoadipate aminotransferase 1 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 503 amino acids
Theoretical weight: 56.57 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P53090 (Residues: 1-500; Coverage: 100%)
Gene names: ARO8, YGL202W
Sequence domains: Aminotransferase class I and II
Structure domains: Type I PLP-dependent aspartate aminotransferase-like (Major domain)
Aromatic/aminoadipate aminotransferase 1 Chain: D
Molecule details ›
Chain: D
Length: 503 amino acids
Theoretical weight: 56.8 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P53090 (Residues: 1-500; Coverage: 100%)
Gene names: ARO8, YGL202W
Sequence domains: Aminotransferase class I and II
Structure domains: Type I PLP-dependent aspartate aminotransferase-like (Major domain)

Ligands and Environments


Cofactor: Ligand PLP 1 x PLP

Cofactor: Ligand PMP 2 x PMP
1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: C2
Unit cell:
a: 227.442Å b: 159.831Å c: 70.642Å
α: 90° β: 106.03° γ: 90°
R-values:
R R work R free
0.184 0.182 0.218
Expression system: Escherichia coli BL21(DE3)