4jdd

X-ray diffraction
2.1Å resolution

14-3-3 protein interaction with Estrogen Receptor Alpha provides a novel drug target interface

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-136921 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
14-3-3 protein sigma Chain: A
Molecule details ›
Chain: A
Length: 236 amino acids
Theoretical weight: 26.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P31947 (Residues: 1-231; Coverage: 93%)
Gene names: HME1, SFN
Sequence domains: 14-3-3 protein
Structure domains: 14-3-3 domain
Estrogen receptor Chain: B
Molecule details ›
Chain: B
Length: 11 amino acids
Theoretical weight: 1.16 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P03372 (Residues: 585-595; Coverage: 2%)
Gene names: ESR, ESR1, NR3A1

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: C2221
Unit cell:
a: 82.49Å b: 111.43Å c: 62.24Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.192 0.232
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided