4jaa

X-ray diffraction
2.39Å resolution

Factor inhibiting HIF-1 alpha in complex with consensus ankyrin repeat domain-(d)LEU peptide

Released:
Source organism: Homo sapiens
Entry authors: Scotti JS, Ge W, McDonough MA, Schofield CJ

Function and Biology Details

Reactions catalysed:
[Ankyrin-repeat domain protein]-L-asparagine + 2-oxoglutarate + O(2) = [ankyrin-repeat domain protein]-(3S)-3-hydroxy-L-asparagine + succinate + CO(2) 
Hypoxia-inducible factor-L-asparagine + 2-oxoglutarate + O(2) = hypoxia-inducible factor-(3S)-3-hydroxy-L-asparagine + succinate + CO(2)
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
Non-polymer only dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Hypoxia-inducible factor 1-alpha inhibitor Chain: A
Molecule details ›
Chain: A
Length: 349 amino acids
Theoretical weight: 40.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NWT6 (Residues: 1-349; Coverage: 100%)
Gene names: FIH1, HIF1AN
Sequence domains: Cupin-like domain
Structure domains:
CONSENSUS ANKYRIN REPEAT DOMAIN-(d)LEU Chain: S
Molecule details ›
Chain: S
Length: 20 amino acids
Theoretical weight: 2.23 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

3 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P41212
Unit cell:
a: 86.079Å b: 86.079Å c: 147.029Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.184 0.219
Expression systems:
  • Escherichia coli
  • Not provided