4j6s

X-ray diffraction
3.08Å resolution

14-3-3gamma complexed with the N-terminal sequence of tyrosine hydroxylase (residues 1-43)

Released:

Function and Biology Details

Reaction catalysed:
L-tyrosine + tetrahydrobiopterin + O(2) = L-dopa + 4a-hydroxytetrahydrobiopterin

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
14-3-3 protein gamma Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 255 amino acids
Theoretical weight: 29.17 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61981 (Residues: 2-247; Coverage: 100%)
Gene name: YWHAG
Sequence domains: 14-3-3 protein
Structure domains: 14-3-3 domain
Tyrosine 3-monooxygenase Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 43 amino acids
Theoretical weight: 4.86 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P07101 (Residues: 1-74; Coverage: 8%)
  • Best match: P07101-6 (Residues: 1-43)
Gene names: TH, TYH
Sequence domains: Tyrosine hydroxylase N terminal

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P212121
Unit cell:
a: 84.19Å b: 115.12Å c: 136.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.21 0.254
Expression systems:
  • Escherichia coli
  • Not provided