› 4j09

X-ray diffraction
1.9Å resolution

Crystal Structure of LpxA bound to RJPXD33

Released:
DOI: 10.2210/pdb4j09/pdb
PDB entry 4j09 coloured by chain, front view.
PDB entry 4j09 coloured by chain, side view.
PDB entry 4j09 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structural basis for the recognition of peptide RJPXD33 by acyltransferases in lipid A biosynthesis.
Jenkins RJ, Heslip KA, Meagher JL, Stuckey JA, Dotson GD
J Biol Chem 289 15527-35 (2014)
PMID: 24742680

Function and Biology Details

Reaction catalysed: 
A (3R)-3-hydroxyacyl-[acyl-carrier-protein] + UDP-N-acetyl-alpha-D-glucosamine = an [acyl-carrier-protein] + a UDP-3-O-(3-hydroxyacylyl)-N-acetyl-alpha-D-glucosamine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141514 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase Chain: A
Molecule details ›
Chain: A
Length: 262 amino acids
Theoretical weight: 28.12 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A722 (Residues: 1-262; Coverage: 100%)
Gene names: JW0176, b0181, lpxA
Sequence domains:
Structure domains:
Sialic acid transporter NanX Chain: B
Molecule details ›
Chain: B
Length: 6 amino acids
Theoretical weight: 754 Da
Source organism: Escherichia coli K-12
Expression system: Not provided
UniProt:
  • Canonical: P39352 (Residues: 101-106; Coverage: 2%)
Gene names: JW5768, b4279, nanX, yjhB

Ligands and Environments

3 bound ligands:
Ligand PO4 3 x PO4
Ligand DMS 1 x DMS
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P213
Unit cell:
a: 96.399Å b: 96.399Å c: 96.399Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.172 0.177
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

3 review citations

Drug discovery strategies to outer membrane targets in Gram-negative pathogens.
Brown DG. (2016)
2 more

3 mentions without citation

Structure, inhibition, and regulation of essential lipid A enzymes.
Zhou et al. (2017)
2 more