4ixo

X-ray diffraction
2.2Å resolution

X-ray structure of NifS-like protein from Rickettsia africae ESF-5

Released:
Source organism: Rickettsia africae ESF-5
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed:
(1a) L-cysteine + [enzyme]-cysteine = L-alanine + [enzyme]-S-sulfanylcysteine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cysteine desulfurase Chains: A, B
Molecule details ›
Chains: A, B
Length: 380 amino acids
Theoretical weight: 41.71 KDa
Source organism: Rickettsia africae ESF-5
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C3PNQ7 (Residues: 1-372; Coverage: 100%)
Gene names: RAF_ORF0675, spl1
Sequence domains: Aminotransferase class-V
Structure domains:

Ligands and Environments

2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P21
Unit cell:
a: 57.02Å b: 91.02Å c: 72.62Å
α: 90° β: 93.72° γ: 90°
R-values:
R R work R free
0.164 0.162 0.205
Expression system: Escherichia coli BL21(DE3)