Structure analysis

A Y37F mutant of C.Esp1396I bound to its highest affinity operator site OM

X-ray diffraction
3.1Å resolution
Source organism: Enterobacter sp. RFL1396
Assembly composition:
hetero tetramer (preferred)
Entry contents: 1 distinct polypeptide molecule
2 distinct DNA molecules

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero tetramer
Accessible surface area: 12300 Å2
Buried surface area: 6700 Å2
Dissociation area: 1,500 Å2
Dissociation energy (ΔGdiss): 21 kcal/mol
Dissociation entropy (TΔSdiss): 11 kcal/mol
Interface energy (ΔGint): -46 kcal/mol
Symmetry number: 1
Assembly 2
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Multimeric state: hetero tetramer

Binding statistics and energies are not available for this assembly

Macromolecules

Chains: A, B, E, F
Length: 82 amino acids
Theoretical weight: 9.51 KDa
Source organism: Enterobacter sp. RFL1396
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8GGH0 (Residues: 1-79; Coverage: 100%)
Gene name: esp1396IC
Pfam: Helix-turn-helix
InterPro:
CATH: lambda repressor-like DNA-binding domains

Search similar proteins

Name: DNA (5'-D(*AP*TP*GP*TP*AP*GP*AP*CP*TP*AP*TP*AP*GP*TP*CP*GP*AP*CP*A)-3')
Representative chains: C, G
Source organism: Enterobacter sp. RFL1396 [211595]
Expression system: Not provided
Length: 19 nucleotides
Theoretical weight: 5.85 KDa

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Name: DNA (5'-D(*TP*TP*GP*TP*CP*GP*AP*CP*TP*AP*TP*AP*GP*TP*CP*TP*AP*CP*A)-3')
Representative chains: D, H
Source organism: Enterobacter sp. RFL1396 [211595]
Expression system: Not provided
Length: 19 nucleotides
Theoretical weight: 5.79 KDa

Search similar DNA