› 4iuq

X-ray diffraction
2.81Å resolution

crystal structure of SHH1 SAWADEE domain

Released:
DOI: 10.2210/pdb4iuq/pdb
PDB entry 4iuq coloured by chain, front view.
PDB entry 4iuq coloured by chain, side view.
PDB entry 4iuq coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Polymerase IV occupancy at RNA-directed DNA methylation sites requires SHH1.
Law JA, Du J, Hale CJ, Feng S, Krajewski K, Palanca AM, Strahl BD, Patel DJ, Jacobsen SE
Nature 498 385-9 (2013)
PMID: 23636332

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195025 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein SAWADEE HOMEODOMAIN HOMOLOG 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 135 amino acids
Theoretical weight: 15.78 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9XI47 (Residues: 125-258; Coverage: 52%)
Gene names: At1g15215, DTF1, F9L1.16, SHH1
Sequence domains: SAWADEE domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand CVM 2 x CVM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21212
Unit cell:
a: 54.708Å b: 117.691Å c: 53.409Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.201 0.261
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

58 review citations

RNA-directed DNA methylation: an epigenetic pathway of increasing complexity.
Matzke et al. (2014)
57 more

1 mention without citation

Polymerase IV occupancy at RNA-directed DNA methylation sites requires SHH1.
Law et al. (2013)