PDBe 4isn

X-ray diffraction
2.45Å resolution

Crystal Structure of Matriptase in complex with its inhibitor HAI-1

Released:

Function and Biology Details

Reaction catalysed:
Cleaves various synthetic substrates with Arg or Lys at the P1 position and prefers small side-chain amino acids, such as Ala and Gly, at the P2 position
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Kunitz-type protease inhibitor 1 Chain: B
Molecule details ›
Chain: B
Length: 62 amino acids
Theoretical weight: 7.1 KDa
Source organism: Homo sapiens
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: O43278 (Residues: 245-305; Coverage: 12%)
  • Best match: O43278-2 (Residues: 245-306)
Gene names: HAI1, SPINT1, UNQ223/PRO256
Sequence domains: Kunitz/Bovine pancreatic trypsin inhibitor domain
Structure domains: Pancreatic trypsin inhibitor Kunitz domain
Suppressor of tumorigenicity 14 protein Chain: A
Molecule details ›
Chain: A
Length: 241 amino acids
Theoretical weight: 26.48 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q9Y5Y6 (Residues: 615-855; Coverage: 28%)
Gene names: PRSS14, SNC19, ST14, TADG15
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P43212
Unit cell:
a: 64.529Å b: 64.529Å c: 172.386Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.21 0.258
Expression systems:
  • Drosophila melanogaster
  • Komagataella pastoris