4irc

X-ray diffraction
2.67Å resolution

Polymerase-DNA complex

Released:

Function and Biology Details

Reaction catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct DNA molecules
Macromolecules (4 distinct):
DNA polymerase IV Chains: A, F
Molecule details ›
Chains: A, F
Length: 342 amino acids
Theoretical weight: 38.3 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: Q47155 (Residues: 2-341; Coverage: 97%)
Gene names: JW0221, b0231, dinB, dinP
Sequence domains:
Structure domains:
DNA (5'-D(*TP*CP*TP*GP*GP*GP*GP*TP*CP*CP*TP*AP*GP*GP*AP*CP*CP*C)-3') Chains: B, G
Molecule details ›
Chains: B, G
Length: 18 nucleotides
Theoretical weight: 5.51 KDa
Source organism: Escherichia coli K-12
Expression system: Not provided
DNA (5'-D(*GP*GP*GP*TP*CP*CP*TP*AP*GP*GP*AP*CP*CP*C)-3') Chain: H
Molecule details ›
Chain: H
Length: 14 nucleotides
Theoretical weight: 4.28 KDa
Source organism: Escherichia coli K-12
Expression system: Not provided
DNA (5'-D(*CP*TP*AP*GP*GP*GP*TP*CP*CP*TP*AP*GP*GP*AP*CP*CP*C)-3') Chain: C
Molecule details ›
Chain: C
Length: 17 nucleotides
Theoretical weight: 5.19 KDa
Source organism: Escherichia coli K-12
Expression system: Not provided

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P21
Unit cell:
a: 86.14Å b: 56.95Å c: 110.81Å
α: 90° β: 93.53° γ: 90°
R-values:
R R work R free
0.227 0.225 0.275
Expression systems:
  • Escherichia coli
  • Not provided