› 4ilu

X-ray diffraction
2.3Å resolution

Crystal structure of Mycobacterium tuberculosis CarD

Released:
DOI: 10.2210/pdb4ilu/pdb
PDB entry 4ilu coloured by chain, front view.
PDB entry 4ilu coloured by chain, side view.
PDB entry 4ilu coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Crystal structure of Mycobacterium tuberculosis CarD, an essential RNA polymerase binding protein, reveals a quasidomain-swapped dimeric structural architecture.
Kaur G, Dutta D, Thakur KG
Proteins 82 879-84 (2014)
PMID: 24115125

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161689 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA polymerase-binding transcription factor CarD Chain: A
Molecule details ›
Chain: A
Length: 170 amino acids
Theoretical weight: 18.99 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WJG3 (Residues: 1-162; Coverage: 100%)
Gene names: Rv3583c, carD
Sequence domains:
Structure domains: CarD-like, C-terminal domain

Ligands and Environments

1 bound ligand:
Ligand SO4 7 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P41212
Unit cell:
a: 93.5Å b: 93.5Å c: 86.51Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.207 0.233
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Legume genomics and transcriptomics: From classic breeding to modern technologies.
Afzal et al. (2020)
11 other articles

2 mentions without citation

Structural insights into RNA polymerase recognition and essential function of Myxococcus xanthus CdnL.
Gallego-García et al. (2014)
1 more