4ilm

X-ray diffraction
3.07Å resolution

CRISPR RNA Processing endoribonuclease

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
CRISPR-associated endoribonuclease Cas6 2 Chains: A, B, D, F, H, J, L, N
Molecule details ›
Chains: A, B, D, F, H, J, L, N
Length: 289 amino acids
Theoretical weight: 32.68 KDa
Source organism: Saccharolobus solfataricus P2
Expression system: Escherichia coli
UniProt:
  • Canonical: Q97WV8 (Residues: 1-289; Coverage: 100%)
Gene names: SSO2004, cas6b
Sequence domains:
Structure domains: Alpha-Beta Plaits
RNA (5'-R(*GP*CP*UP*AP*AP*UP*CP*UP*AP*CP*UP*AP*UP*AP*GP*A)-3') Chains: C, E, G, I, K, M, O, R
Molecule details ›
Chains: C, E, G, I, K, M, O, R
Length: 16 nucleotides
Theoretical weight: 5.07 KDa
Source organism: Saccharolobus solfataricus P2
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 78.981Å b: 154.608Å c: 130.848Å
α: 90° β: 93.64° γ: 90°
R-values:
R R work R free
0.235 0.233 0.284
Expression systems:
  • Escherichia coli
  • Not provided