4il0

X-ray diffraction
2.8Å resolution

Crystal structure of GlucDRP from E. coli K-12 MG1655 (EFI target EFI-506058)

Released:
Entry authors: Lukk T, Ghasempur S, Imker HJ, Gerlt JA, Nair SK, Enzyme Function Initiative (EFI)

Function and Biology Details

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucarate dehydratase-related protein Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 466 amino acids
Theoretical weight: 51.08 KDa
Source organism: Escherichia coli str. K-12 substr. MG1655
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q46915 (Residues: 1-446; Coverage: 100%)
Gene names: JW2759, b2788, gudX, ygcY
Sequence domains: Mandelate racemase / muconate lactonizing enzyme, N-terminal domain
Structure domains:

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P1
Unit cell:
a: 86.09Å b: 94.03Å c: 155.2Å
α: 101.47° β: 96.74° γ: 79.86°
R-values:
R R work R free
0.226 0.173 0.231
Expression system: Escherichia coli BL21(DE3)