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High resolution structure of GCaMP3 dimer form 1 at pH 7.5

X-ray diffraction
1.55Å resolution
PDB entry 4ik8 coloured by chain, front view.
PDB entry 4ik8 coloured by chain, side view.
PDB entry 4ik8 coloured by chain, top view.
Source organisms:
Assembly composition:
homo dimer (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: EF-hand domain-containing protein, Green fluorescent protein
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Multimeric state: homo dimer
Accessible surface area: 35280.1 Å2
Buried surface area: 8049.43 Å2
Dissociation area: 3,676.21 Å2
Dissociation energy (ΔGdiss): 56.92 kcal/mol
Dissociation entropy (TΔSdiss): 15.12 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-125521

Macromolecules

EF-hand domain-containing protein; Green fluorescent protein
Chain: A
Length: 448 amino acids
Theoretical weight: 50.54 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: K4DIE3 (Residues: 1-48, 51-59, 60-94, 95-127, 128-147, 279-283, 306-338, 342-354, 361-362, 368-432; Coverage: 58%)
  • Canonical: P42212 (Residues: 2-141, 145-170, 185-187, 201-206, 209-213; Coverage: 74%)
    • nullnull
Gene name: GFP
Pfam:
InterPro:
CATH: Green fluorescent protein
EF-hand domain-containing protein in PDB entry 4ik8, assembly 1, front view.
EF-hand domain-containing protein in PDB entry 4ik8, assembly 1, side view.
EF-hand domain-containing protein in PDB entry 4ik8, assembly 1, top view.
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