› 4ij0

X-ray diffraction
1.54Å resolution

Structures of DNA duplexes containing O6-carboxymethylguanine, a lesion associated with gastrointestinal cancer, reveal a mechanism for inducing transition mutation

Released:
DOI: 10.2210/pdb4ij0/pdb
PDB entry 4ij0 coloured by chain, front view.
PDB entry 4ij0 coloured by chain, side view.
PDB entry 4ij0 coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
Structures of DNA duplexes containing O6-carboxymethylguanine, a lesion associated with gastrointestinal cancer, reveal a mechanism for inducing pyrimidine transition mutations.
Zhang F, Tsunoda M, Suzuki K, Kikuchi Y, Wilkinson O, Millington CL, Margison GP, Williams DM, Czarina Morishita E, Takénaka A
Nucleic Acids Res 41 5524-32 (2013)
PMID: 23580550

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117649 (preferred)
Entry contents:
1 distinct DNA molecule
Macromolecule:
DNA (5'-D(*CP*GP*CP*(C6G)P*AP*AP*TP*TP*CP*GP*CP*G)-3') Chains: A, B
Molecule details ›
Chains: A, B
Length: 12 nucleotides
Theoretical weight: 3.72 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand SR 3 x SR
Ligand HT 1 x HT
1 modified residue:
Ligand C6G 2 x C6G

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P212121
Unit cell:
a: 24.6Å b: 41.23Å c: 63.07Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.194 0.237
Expression system: Not provided

Quick links

Citations

1 review citation

O6-methylguanine-DNA methyltransferase in the defense against N-nitroso compounds and colorectal cancer.
Fahrer et al. (2013)
4 other articles

1 mention without citation

Structures of DNA duplexes containing O6-carboxymethylguanine, a lesion associated with gastrointestinal cancer, reveal a mechanism for inducing pyrimidine transition mutations.
Zhang et al. (2013)