4i27

X-ray diffraction
2.6Å resolution

Ternary complex of mouse TdT with ssDNA and incoming nucleotide

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
DNA nucleotidylexotransferase Chain: A
Molecule details ›
Chain: A
Length: 400 amino acids
Theoretical weight: 45.7 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P09838 (Residues: 132-510; Coverage: 74%)
Gene names: Dntt, Tdt
Sequence domains:
Structure domains:
5'-D(*AP*AP*AP*AP*AP*T)-3' Chain: C
Molecule details ›
Chain: C
Length: 6 nucleotides
Theoretical weight: 1.81 KDa
Source organism: Mus musculus
Expression system: Not provided

Ligands and Environments

3 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P21
Unit cell:
a: 46.94Å b: 95.9Å c: 47.23Å
α: 90° β: 107.75° γ: 90°
R-values:
R R work R free
0.174 0.171 0.233
Expression systems:
  • Escherichia coli
  • Not provided