4i13

X-ray diffraction
1.6Å resolution

Nanobody ca1697 binding to the DHFR.folate binary complex

Released:
Entry authors: Oyen D, Steyaert J, Barlow JN

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Dihydrofolate reductase Chain: A
Molecule details ›
Chain: A
Length: 159 amino acids
Theoretical weight: 18.02 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ABQ4 (Residues: 1-159; Coverage: 100%)
Gene names: JW0047, b0048, folA, tmrA
Sequence domains: Dihydrofolate reductase
Structure domains: Dihydrofolate Reductase, subunit A
Protein ca1697 (nanobody) Chain: B
Molecule details ›
Chain: B
Length: 133 amino acids
Theoretical weight: 14.83 KDa
Source organism: Lama glama
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P212121
Unit cell:
a: 49.76Å b: 58.05Å c: 114.38Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.155 0.154 0.18
Expression system: Escherichia coli