› 4hhd

X-ray diffraction
2.75Å resolution

2.75 Angstrom resolution crystal structure of the A. thaliana LOV2 domain with an extended N-terminal A' helix (cryo dark structure)

Released:
DOI: 10.2210/pdb4hhd/pdb
PDB entry 4hhd coloured by chain, front view.
PDB entry 4hhd coloured by chain, side view.
PDB entry 4hhd coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Coiled-coil dimerization of the LOV2 domain of the blue-light photoreceptor phototropin 1 from Arabidopsis thaliana.
Halavaty AS, Moffat K
Acta Crystallogr Sect F Struct Biol Cryst Commun 69 1316-21 (2013)
PMID: 24316821

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129257 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phototropin-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 166 amino acids
Theoretical weight: 19.12 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O48963 (Residues: 452-615; Coverage: 17%)
Gene names: At3g45780, JK224, NPH1, PHOT1, RPT1, T6D9_110
Sequence domains: PAS domain
Structure domains: PAS domain

Ligands and Environments


Cofactor: Ligand FMN 2 x FMN
1 bound ligand:
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P41212
Unit cell:
a: 63.382Å b: 63.382Å c: 171.318Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.231 0.289
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Plant flavoprotein photoreceptors.
Christie et al. (2015)
4 more

7 mentions without citation

Setting the PAS, the role of circadian PAS domain proteins during environmental adaptation in plants.
Vogt et al. (2015)
6 more