4hh1

X-ray diffraction
3.5Å resolution

Dark-state structure of AppA wild-type without the Cys-rich region from Rb. sphaeroides

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
BLUF domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 406 amino acids
Theoretical weight: 43.88 KDa
Source organism: Cereibacter sphaeroides
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q53119 (Residues: 3-399; Coverage: 88%)
Gene name: appA
Sequence domains: Sensors of blue-light using FAD
Structure domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P3212
Unit cell:
a: 70.53Å b: 70.53Å c: 383.81Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.188 0.186 0.226
Expression system: Escherichia coli BL21(DE3)