› 4hh1

X-ray diffraction
3.5Å resolution

Dark-state structure of AppA wild-type without the Cys-rich region from Rb. sphaeroides

Released:
DOI: 10.2210/pdb4hh1/pdb
PDB 4hh1 coloured by chain and viewed from the front.
PDB 4hh1 coloured by chain and viewed from the side.
PDB 4hh1 coloured by chain and viewed from the top.
Source organism: Cereibacter sphaeroides
Primary publication:
A ternary AppA-PpsR-DNA complex mediates light regulation of photosynthesis-related gene expression.
Winkler A, Heintz U, Lindner R, Reinstein J, Shoeman RL, Schlichting I
OpenAccess logo Nat Struct Mol Biol 20 859-67 (2013)
PMID: 23728293

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
BLUF domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 406 amino acids
Theoretical weight: 43.88 KDa
Source organism: Cereibacter sphaeroides
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q53119 (Residues: 3-399; Coverage: 88%)
Gene name: appA
Sequence domains: Sensors of blue-light using FAD
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand FMN 2 x FMN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P3212
Unit cell:
a: 70.53Å b: 70.53Å c: 383.81Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.188 0.186 0.226
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

Optophysiology: Illuminating cell physiology with optogenetics.
Tan et al. (2022)
9 more

5 mentions without citation

Seeing the light with BLUF proteins.
Park et al. (2017)
4 more