› 4hfo

X-ray diffraction
3Å resolution

Biogenic amine-binding protein selenomethionine derivative

Released:
DOI: 10.2210/pdb4hfo/pdb
PDB entry 4hfo coloured by chain, front view.
PDB entry 4hfo coloured by chain, side view.
PDB entry 4hfo coloured by chain, top view.
Source organism: Rhodnius prolixus
Primary publication:
Structure and ligand-binding properties of the biogenic amine-binding protein from the saliva of a blood-feeding insect vector of Trypanosoma cruzi.
Xu X, Chang BW, Mans BJ, Ribeiro JM, Andersen JF
Acta Crystallogr D Biol Crystallogr 69 105-13 (2013)
PMID: 23275168

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183140 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitrophorin domain-containing protein Chains: A, B, C, D, I, J, K, L
Molecule details ›
Chains: A, B, C, D, I, J, K, L
Length: 195 amino acids
Theoretical weight: 22.17 KDa
Source organism: Rhodnius prolixus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q86PT9 (Residues: 23-217; Coverage: 100%)
Sequence domains: Nitrophorin
Structure domains: Lipocalin

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 16 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 102.164Å b: 70.661Å c: 108.413Å
α: 90° β: 99.16° γ: 90°
R-values:
R R work R free
0.322 0.321 0.349
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Insights into the sand fly saliva: Blood-feeding and immune interactions between sand flies, hosts, and Leishmania.
Lestinova et al. (2017)
1 more

1 mention without citation

Structure and ligand-binding properties of the biogenic amine-binding protein from the saliva of a blood-feeding insect vector of Trypanosoma cruzi.
Xu et al. (2013)