4hel

X-ray diffraction
3.2Å resolution

Crystal structure analysis of apo-GroEL structure

Released:
Source organism: Escherichia coli
Entry authors: Saxena AK, Meena SR

Function and Biology Details

Reaction catalysed:
ATP + H(2)O + a folded polypeptide = ADP + phosphate + an unfolded polypeptide
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetradecamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chaperonin GroEL Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N
Length: 525 amino acids
Theoretical weight: 55.35 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: Q548M1 (Residues: 2-526; Coverage: 96%)
Gene names: A5U30_003693, A8499_001284, A8499_005479, A9X72_22960, ACN68_26960, ACN81_06325, ACU57_19295, AF998_003319, AF998_005338, AM464_15770, APX88_10225, AT335_004348, AT845_002714, AW118_06985, AW119_22780, B6R12_001246, B6R12_005482, B6R15_002033, B6R31_000859, B6R48_001160, B6R87_001161, BANRA_00147, BANRA_00718, BEA19_16435, BER14_11410, BF481_004183, BG944_005198, BGM66_001100, BHS81_24830, BJI68_16795, BJJ90_23450, BK292_23445, BK383_12940, BKL28_003536, BKL28_005489, BLM69_001195, BMC79_004320, BMC79_005357, BMT49_19180, BMT50_06320, BMT91_05495, BN17_41231, BO068_004278, BOH76_02880, BON63_07075, BON64_05055, BON65_22610, BON66_25750, BON67_00070, BON68_27655, BON69_04690, BON70_25350, BON71_15855, BON72_19590, BON73_07370, BON74_00810, BON75_25260, BON76_04275, BON77_18135, BON78_14675, BON79_10870, BON80_13640, BON81_15965, BON82_09980, BON83_17240, BON84_18355, BON86_07405, BON87_11955, BON88_05980, BON90_19910, BON91_12970, BON92_18275, BON93_15345, BON94_08655, BON95_05420, BON96_03715, BON97_19710, BON98_17880, BR158_004302, BR158_005676, BRV02_002050, BSR05_14030, BTB68_004143, BTQ06_07135, BUO55_004105, BVL39_04415, BXT93_23590, BZL69_06910, BvCmsF30A_00833, BvCmsHHP019_00806, BvCmsHHP056_01305, BvCmsKKP036_00132, BvCmsKKP061_03324, BvCmsKSNP073_03447, C0P57_003473, C1Q91_002277, C2121_001093, C2121_005324, C2M16_07145, C2R31_001785, C2R31_005321, C2U48_11800, C3F40_14340, C5N07_07010, C5Y87_09545, C9114_00680, C9160_20130, C9E67_27735, C9Z68_08425, CA593_05105, CCS08_01935, CCV12_000687, CCV12_005017, CDC27_22515, CDL36_17910, CDL37_19955, CF22_001662, CF22_005318, CG831_003422, CHM61_000620, CIG67_06440, CO706_20635, CQ986_004336, CQ986_005711, CR539_02660, CR628_000792, CV83915_01888, CWS33_03390, CXJ73_002181, CY655_26170, D0X26_12780, D3822_10750, D3C88_27260, D3G36_16020, D3G36_27385, D3Y67_00555, D4N09_04290, D4U49_20275, D4U49_28470, D9D43_14725, D9D77_08895, D9E34_15015, D9E49_11975, D9H94_06455, D9J03_08605, D9J61_09370, DAH17_10445, DAH18_16415, DAH19_12055, DAH20_11960, DAH21_25315, DAH22_18760, DAH23_05700, DAH24_05050, DAH25_03370, DAH26_05045, DAH27_28010, DAH28_27455, DAH29_17635, DAH30_16205, DAH31_24530, DAH34_17585, DAH35_18130, DAH36_09875, DAH37_06370, DAH38_24275, DAH40_25495, DAH41_16405, DAH42_13205, DAH43_08635, DAH45_08870, DAH46_08620, DAH47_07725, DAH48_07865, DAH49_08645, DAH50_08530, DD762_21030, DEN86_00710, DEN87_24800, DEN88_19475, DEN89_17490, DEN90_13130, DEN91_19945, DEN92_15550, DEN93_06415, DEN94_10790, DEN95_17440, DEN96_12410, DEN97_13360, DEN98_15280, DEN99_13325, DEO00_11350, DEO01_13545, DEO02_24175, DEO03_06950, DEO04_24535, DEO05_24405, DEO06_14135, DEO07_12755, DEO08_16810, DEO09_24950, DEO10_24520, DEO11_24170, DEO12_13985, DEO13_05685, DEO14_14875, DEO15_07710, DEO17_15430, DEO18_20465, DEO19_26325, DEO20_25075, DKP82_04065, DM870_10425, DN627_22730, DNQ45_22695, DNX30_08345, DNX30_30410, DRW19_22655, DS732_02495, DTL43_02185, DTL90_17410, DTM45_21115, DU321_10255, DXT69_20075, DXT70_06700, DXT71_04100, DXT73_07230, E0I42_01220, E2112_14800, E2113_05120, E2114_01880, E2115_03725, E2116_03515, E2117_08600, E2118_02080, E2120_05935, E2121_02935, E2122_03135, E2123_02135, E2124_07020, E2125_03525, E2127_07020, E2128_02070, E2129_04150, E2130_03760, E2132_24265, E2133_02315, E2134_13845, E2136_01870, E2646_23210, E3N34_15825, E4K51_08915, E4T14_12645, E4T84_12320, E5H86_25825, E5M02_22815, E5P22_06470, E5P23_06190, E5P24_10765, E5P25_11670, E5P26_01745, E5P27_12285, E5P28_03065, E5P29_11580, E5P30_19990, E5P31_11265, E5P32_03385, E5P33_11365, E5P34_20020, E5P35_07385, E5P36_08610, E5P37_09765, E5P39_02480, E5P40_16785, E5P41_06165, E5P42_03115, E5P43_03105, E5P44_08720, E5P45_01355, E5P46_19000, E5P47_03005, E5P48_09930, E5P49_08945, E5P50_02825, E5P51_05670, E5P52_11560, E5S34_00685, E5S35_05300, E5S36_08480, E5S37_06420, E5S38_10295, E5S39_12665, E5S42_15075, E5S43_14760, E5S44_14045, E5S45_06335, E5S46_10410, E5S47_14980, E5S48_04730, E5S51_06565, E5S52_04995, E5S53_13220, E5S54_12890, E5S55_12185, E5S57_19130, E5S58_09490, E5S59_12615, E5S61_14350, E5S62_09630, E6C80_20905, E6D34_03900, EA239_16730, EA435_16360, EAI46_05875, EAN77_04200, EAN77_30790, EAX79_03060, EBP16_10875, EC1094V2_4111, EC95NR1_03760, ECs5124, EHD79_25330, EI021_09520, EIA08_20145, EKI52_12830, EL79_4200, EL80_4116, ELT17_14070, ELT20_09305, ELT21_01005, ELT22_11950, ELT24_09420, ELT25_09435, ELT26_14250, ELT27_08410, ELT28_08405, ELT29_19010, ELT30_18130, ELT31_17770, ELT33_14955, ELT34_11390, ELT35_22660, ELT36_09055, ELT39_09770, ELT40_14710, ELT41_02385, ELT44_17185, ELT45_11555, ELT46_12890, ELT48_15395, ELT50_17565, ELT51_08255, ELT52_15970, ELT54_04355, ELT55_10320, ELT56_16145, ELT58_06705, ELT59_12910, ELT60_17010, ELT61_04400, ELT63_14055, ELT72_09970, ELU07_20900, ELU82_07640, ELU83_14270, ELU85_14365, ELU88_11140, ELU89_09850, ELU90_16290, ELU91_13705, ELU94_20525, ELU95_17870, ELU96_21405, ELU97_18775, ELU98_18375, ELU99_11245, ELV00_11890, ELV01_16465, ELV02_00870, ELV03_16485, ELV04_14305, ELV05_12715, ELV08_11530, ELV09_04460, ELV10_15595, ELV11_06260, ELV12_04820, ELV13_23125, ELV15_01140, ELV16_04385, ELV20_04115, ELV21_14030, ELV22_09270, ELV23_19700, ELV26_07245, ELV28_07840, ELV29_15715, ELV40_01565, ELX48_07490, ELX61_05655, ELX66_12445, ELX68_21125, ELX69_13310, ELX76_24925, ELX79_22305, ELX85_01345, ELX96_02710, ELY02_12210, ELY31_07465, ELY32_09185, ELY36_08775, ELY39_09530, ELY41_00375, ELY48_02650, EN85_000862, EPS76_03470, ERS139208_00817, EWK56_08835, EXX13_20070, EYV17_07185, EYV18_13170, EYX47_03680, ExPECSC038_04651, F0L67_00815, F2N20_19365, F2N31_14635, F3N40_18140, F7F11_04220, F7N46_18305, F7N46_27130, F9413_14230, F9461_18160, F9461_28440, F9B07_06155, F9S83_12790, F9S83_28070, F9V24_14020, F9X20_07075, FA868_05635, FA868_23545, FDM60_19580, FE584_02505, FE587_02500, FE587_27615, FEJ01_03285, FEJ01_27320, FFF58_08400, FGG80_08295, FHO90_11795, FHO90_28775, FHQ91_05695, FHQ91_27655, FIJ20_10460, FJQ40_01790, FJQ51_04800, FJQ51_27630, FJQ53_01090, FJQ53_27560, FKO60_18130, FOI11_014910, FOI11_20770, FPI65_25535, FPJ29_22130, FPS11_04035, FPS11_29800, FTV92_15760, FV293_00695, FVB16_06060, FY127_22265, FZC17_10050, FZN31_22125, FZU14_15570, G3565_26040, G3813_002809, G3813_005845, G3V95_22465, G3W53_08890, G4A38_02930, G4A47_11060, G7630_000779, G7630_005557, G9448_12350, GAI66_22455, GAI89_04545, GAJ12_10905, GAJ26_12690, GF699_21755, GFY34_22220, GFY34_26140, GJ11_26030, GJO56_12545, GKF66_07975, GKF89_03295, GNW61_00495, GNZ05_11940, GOP25_14290, GP711_17780, GP944_08950, GP954_08230, GP975_02890, GQM04_14160, GQM13_10590, GQM21_05675, GQN34_02395, GQW07_07140, GQW07_26595, GQW68_02145, GQW68_27055, GQW80_12405, GQW80_27320, GRC73_06265, GRO95_07230, GRO95_26670, GRW05_17640, GRW24_06445, GRW56_03925, GRW57_04440, GSM54_13320, GSM54_27565, GSY44_04690, GSY44_27830, GTP88_02585, GTP88_26745, GUC01_08885, GUI33_10710, H0O37_23780, H0O39_17170, H0O51_02155, H0O72_04755, H6Y26_004443, H6Y26_005600, HEP30_014195, HHH44_003373, HI055_001395, HI055_004873, HIE29_005290, HIN64_003381, HIN64_005174, HJQ60_002547, HJQ60_005759, HJS37_003363, HJS37_005154, HJU54_000770, HJU54_005215, HKA49_000870, HL563_08315, HL601_13705, HL601_27110, HLV18_08720, HLV18_24510, HLX92_12540, HLZ50_12865, HMJ82_15920, HMU06_18255, HMU06_28485, HMV95_06875, HMW38_14420, HMW38_27590, HNC36_09345, HNC59_12710, HNC59_29175, HNC66_09205, HNC66_27645, HNC99_22380, HNC99_29315, HND12_24605, HND12_30370, HV109_21650, HV209_20120, HVV39_15060, HVW04_12630, HVW43_14140, HVY77_23275, HVZ71_22695, HX136_22855, HmCms169_03328, I6H00_16320, I6H01_15150, I6H02_16535, IA00_000538, IA00_005715, IH768_00055, IH772_04800, IT029_000740, IT029_005501, J4S20_000448, J5U05_001408, JE86ST02C_47230, JE86ST05C_48290, JFD_00797, JNP96_02875, NCTC10082_01857, NCTC10089_04560, NCTC10090_02607, NCTC10418_06721, NCTC10764_04513, NCTC10767_00467, NCTC10865_05650, NCTC10974_05136, NCTC11112_02719, NCTC11181_01789, NCTC11341_03019, NCTC12950_04949, NCTC13127_05944, NCTC13216_02274, NCTC4450_01486, NCTC7927_05027, NCTC8008_04112, NCTC8333_05251, NCTC8450_00576, NCTC8500_05095, NCTC8621_04680, NCTC8622_02021, NCTC8959_04316, NCTC8960_02051, NCTC9036_04433, NCTC9037_04611, NCTC9044_02622, NCTC9045_05354, NCTC9073_03984, NCTC9077_05690, NCTC9081_01494, NCTC9111_04720, NCTC9117_05641, NCTC9702_05313, NCTC9706_01822, NCTC9775_02929, NCTC9777_00950, ND22_003151, RG28_06025, SAMEA3472044_01554, SAMEA3472056_01662, SAMEA3751407_02300, SAMEA3753106_03412, TUM18780_39840, WP4S18E07_41060, WR15_07175, XU56_000048, XU56_005610, groEL, groL, groL_1, mopA
Sequence domains: TCP-1/cpn60 chaperonin family
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P212121
Unit cell:
a: 136.24Å b: 261.83Å c: 282.28Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.198 0.223