4hel

X-ray diffraction
3.2Å resolution

Crystal structure analysis of apo-GroEL structure

Released:
Source organism: Escherichia coli
Entry authors: Saxena AK, Meena SR

Function and Biology Details

Structure analysis Details

Assembly composition:
homo tetradecamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
60 kDa chaperonin Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N
Length: 525 amino acids
Theoretical weight: 55.35 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: Q548M1 (Residues: 2-526; Coverage: 96%)
Gene names: A6581_03125, A6V01_27720, A8C65_01580, A9P13_23810, A9R57_13545, A9X72_22960, AC067_05505, AC789_1c45570, ACN002_4372, ACN68_26960, ACN81_06325, ACU57_19295, ACU90_27610, AJ318_17205, AM270_14500, AM446_25370, AM464_15770, AM465_02150, AMK83_27450, AML35_20705, APT94_02245, APZ14_01135, ARC77_17990, AU473_28145, AUQ13_10300, AUS26_18735, AW059_20455, AW106_26290, AWB10_23735, AWE53_002045, AWF59_005875, AWG78_009865, AWG90_025680, B6V57_24240, B7C53_14115, B9M99_07795, B9N33_15905, B9T59_10250, BANRA_00108, BANRA_00147, BANRA_02787, BANRA_02830, BB545_11040, BE963_12230, BEN53_00765, BER14_11410, BFD68_08450, BHF03_14145, BHF46_15625, BHS81_24830, BHS87_23225, BIQ87_23895, BIU72_06095, BIZ41_02710, BJJ90_23450, BK248_23630, BK292_23445, BK296_02280, BK334_08295, BK373_03440, BK375_06855, BK383_12940, BMA87_21445, BMT49_19180, BMT91_05495, BN17_41231, BOH76_02880, BON63_07075, BON65_22610, BON66_25750, BON69_04690, BON71_15855, BON72_19590, BON75_25260, BON76_04275, BON83_17240, BON86_07405, BON87_11955, BON91_12970, BON92_18275, BON94_08655, BON95_05420, BON96_03715, BTQ04_18135, BTQ06_07135, BUE81_24315, BVL39_04415, BW690_22405, BWI89_16475, BWP17_13605, BXT93_23590, BZL31_12950, BZL69_06910, BvCms12BK_04199, BvCms2454_04385, BvCms28BK_01487, BvCms35BK_01935, BvCmsC61A_01711, BvCmsHHP001_00679, BvCmsHHP019_00806, BvCmsHHP056_01305, BvCmsKKP036_00132, BvCmsKKP061_03324, BvCmsKSNP073_03447, BvCmsKSNP081_01939, BvCmsKSNP120_00880, BvCmsKSP011_04701, BvCmsKSP024_01292, BvCmsKSP026_01573, BvCmsKSP045_02508, BvCmsKSP058_02605, BvCmsKSP067_03875, BvCmsNSNP036_02363, BvCmsNSP006_05622, BvCmsNSP007_04896, BvCmsNSP047_02052, BvCmsNSP072_00461, BvCmsOUP014_01585, BvCmsSINP011_01477, BvCmsSINP022_02410, BvCmsSIP019_03074, BvCmsSIP044_04391, C2M16_07145, C2U48_11800, C4K41_15740, C4M78_05800, C5715_24370, C5N07_07010, C5P01_11485, C5P44_18550, C6669_06700, C6B13_11455, C7235_22800, C7B02_22870, C7B06_19620, C7B07_19640, C7B08_13320, C7B18_22125, C9098_11410, C9114_00680, C9141_20510, C9160_20130, C9162_14155, C9201_17520, C9306_22895, C9E25_08280, C9E67_27735, C9Z03_10905, C9Z23_18645, C9Z28_23700, C9Z29_19670, C9Z39_10120, C9Z43_22545, C9Z68_08425, C9Z69_17815, C9Z70_16200, C9Z78_16845, C9Z89_21585, CA593_05105, CCZ14_19960, CCZ17_16290, CDC27_22515, CDL37_19955, CF006_04695, CG692_00055, CI641_014245, CI693_12655, CI694_17140, CIG45_10085, CJU63_24545, CMR93_14020, CO706_20635, COD30_28660, COD46_17610, CQP61_24785, CR539_02660, CRD98_26740, CRE06_15175, CRJUMX01_530009, CRM83_17505, CRT43_24985, CRX46_11625, CSB64_20580, CT146_11195, CUB99_10685, CV83915_01888, CVH05_06685, CWM24_06435, CWS33_03390, D0X26_12780, D2184_07875, D2185_07635, D2188_20735, D3822_10750, D3C88_27260, D3M98_16560, D3O91_01400, D3P01_12765, D3P02_10285, D3Y67_00555, D4011_02335, D4011_28795, D4023_24445, D4074_12335, D4074_28065, D4628_24265, D4636_24995, D4638_20515, D4660_14220, D4660_28105, D4718_12750, D4L91_10435, D4L91_28785, D4M06_10270, D4M06_29410, D4U49_20275, D4U49_28470, D4U85_11010, D4U85_28965, D4V08_09395, D5H35_23635, D5I97_10335, D5I97_29045, D6004_23030, D6C36_13230, D6C36_28445, D6D43_13715, D6D43_29350, D6T60_08370, D6W00_21570, D6X36_01360, D6X63_17145, D6X76_09075, D6X76_29465, D7K33_08230, D7K33_28455, D7K63_02295, D7K66_01645, D7W70_13165, D7Y10_20710, D7Z75_21475, D8Y65_09645, D8Y65_29360, D9610_22370, D9C99_20305, D9D20_08430, D9D31_08050, D9D33_02520, D9D43_14725, D9D44_05930, D9D94_05390, D9E13_11800, D9E19_18585, D9E34_15015, D9E35_09915, D9E49_11975, D9E73_04555, D9F17_02185, D9F32_10570, D9F87_11960, D9G11_21625, D9G42_15395, D9G48_11760, D9G69_11420, D9G95_09380, D9H10_23635, D9H36_10345, D9H53_05445, D9H68_15855, D9H70_08850, D9H94_06455, D9I18_04540, D9I87_11050, D9I88_12295, D9I97_14780, D9J03_08605, D9J44_18175, D9J46_16915, D9J52_05945, D9J58_20605, D9J60_12275, D9J63_12815, D9J78_19045, D9K02_08765, D9K48_05190, D9K54_26350, D9L89_02290, D9L89_29680, D9L99_20610, D9S45_09405, D9X77_11690, D9X77_27245, D9X97_13605, D9X97_28710, D9Z28_19325, DAH18_16415, DAH26_05045, DAH30_16205, DAH34_17585, DAH37_06370, DAH43_08635, DB359_12000, DBQ99_23660, DD762_21030, DEN86_00710, DEN89_17490, DEN97_13360, DEO04_24535, DEO19_26325, DJ487_18360, DJ492_21620, DJ503_21955, DK132_21945, DL251_20250, DL251_25995, DL257_06855, DL257_26035, DL292_23900, DL326_21490, DL455_10645, DL479_22395, DL530_24735, DL545_22730, DL800_29220, DL979_24565, DL979_26215, DLT82_13510, DLT82_28090, DLU50_04890, DLU67_18735, DLU82_20050, DLW60_25690, DLW88_24690, DLW88_28620, DLX38_16350, DLX38_27700, DLX40_20245, DLY41_20340, DLY44_11720, DM102_08870, DM129_07825, DM155_25270, DM267_18730, DM272_20435, DM272_27585, DM280_12770, DM280_26265, DM296_20595, DM382_23740, DM820_21810, DM962_19000, DM973_23780, DMI04_11460, DMI04_28175, DMI53_04370, DMI53_30055, DMO02_14455, DMO02_29440, DMZ50_22485, DMZ50_28115, DN627_22730, DN660_22545, DN700_24935, DN703_08355, DN703_30665, DN808_14005, DNB37_06985, DNB37_28240, DNC98_05725, DND16_08930, DND16_27675, DND79_22010, DNI21_07355, DNJ62_11730, DNJ62_29205, DNK12_12585, DNK12_28175, DNQ45_22695, DNR35_20070, DNR41_18215, DNW42_03440, DNX19_14450, DNX30_08345, DNX30_30410, DOE35_12275, DOE35_31185, DOM23_19100, DOM23_26315, DOS18_11520, DOS18_28020, DOT81_12600, DOT81_29465, DOU81_10025, DOU81_28535, DOY22_05440, DOY56_08400, DOY56_28305, DOY61_23910, DOY67_14955, DP258_24460, DP265_18880, DP277_20350, DQE83_13435, DQE91_24695, DQF36_20915, DQF57_23270, DQF71_26180, DQF72_19220, DQG35_13885, DQG35_28640, DQO13_25440, DQP61_22195, DRP48_12950, DRP48_28250, DRW19_22655, DS143_26430, DS721_10755, DS721_28365, DS732_02495, DS966_08020, DT034_23795, DTL43_02185, DTL90_17410, DTM10_04525, DTM45_21115, DTZ20_23990, DTZ20_30255, DU309_08105, DU321_10255, DU333_08465, DVB38_23740, DW236_18440, DWB25_22555, DXT69_20075, DXT71_04100, DXT73_07230, DXX80_015065, E0I42_01220, E0K84_20485, E0L04_22495, E0L12_16055, E2112_14800, E2114_01880, E2115_03725, E2127_07020, E2128_02070, E2129_04150, E2134_13845, E2148_16540, E2855_05287, E2863_05073, E4K55_08760, E4K60_10770, E4K61_05830, E5P22_06470, E5P28_03065, E5P37_09765, E5S42_15075, E5S46_10410, E5S47_14980, E5S58_09490, E5S61_14350, EA159_10565, EA167_13005, EA189_17960, EA191_15010, EA200_16350, EA203_12885, EA213_12185, EA214_14310, EA218_07230, EA222_18065, EA223_01205, EA225_21165, EA231_14300, EA232_12495, EA233_14040, EA242_18995, EA250_13490, EA410_11360, EA429_17040, EA434_08355, EA435_16360, EA834_13510, EAI42_02210, EAI46_05875, EAI52_08825, EAM59_26250, EAN70_26425, EAN77_04200, EAN77_30790, EAX79_03060, EB476_06015, EB509_04245, EB510_02850, EB515_02245, EB515_27855, EBA46_03220, EBA84_09370, EBA84_28120, EBJ06_01305, EBJ06_28485, EBM08_22005, EC1094V2_4111, EC3234A_108c00480, EC3426_00475, EC382_20320, EC95NR1_03760, ECONIH1_24685, ECTO124_04553, ECTO6_04343, ECs5124, ED225_08645, ED225_27880, ED307_02560, ED600_22995, ED607_16100, ED611_08915, ED903_07130, ED944_02245, ED944_28230, EEA45_03810, EEA45_28005, EEP23_07625, EF082_10500, EF082_28260, EF173_02240, EG075_08115, EG599_24615, EG796_20885, EG808_19065, EGC26_01700, EGC26_27120, EGT48_17625, EGU87_11215, EH186_16900, EH412_25905, EHD45_05145, EHD63_02245, EHD63_27685, EHD79_25330, EHH55_19100, EHJ36_27130, EHJ66_01305, EHJ66_27440, EHV81_05685, EHV81_28700, EHV90_05845, EHV90_28280, EHW09_10550, EHW09_28755, EHX09_11460, EHX09_28360, EI021_09520, EI028_05320, EI032_02755, EI041_04840, EIA08_20145, EIA21_18795, EIZ86_02695, EJ366_20730, EJC75_20460, EKI52_12830, EL75_4022, EL79_4200, EL80_4116, ELT20_09305, ELT33_14955, ELT34_11390, ELT48_15395, ELT58_06705, ELU82_07640, ELU85_14365, ELU96_21405, ELV05_12715, ELV08_11530, ELV13_23125, ELV15_01140, ELV24_17430, ELV28_07840, ELX79_22305, ELX83_07205, ELY05_00695, ELY23_03820, ELY24_10375, ELY50_08540, EO241_15015, EPS76_03470, EPS91_16025, EPS94_17720, EPU41_23390, EQ823_13510, EQ830_14810, ERS085365_01231, ERS085366_02367, ERS085374_01721, ERS085379_04164, ERS085383_01552, ERS085386_01025, ERS085404_01348, ERS085416_03698, ERS139211_04024, ERS150873_01289, ERS150876_03930, EST51_23025, EVY14_04860, EWK56_08835, EXM29_20525, EXX06_20585, EXX13_20070, EXX23_19135, EXX24_09465, EXX53_17255, EXX55_21120, EXX71_14845, EXX78_13730, EXX87_19165, EYD11_20730, EYX82_10180, EYX99_04255, EYY27_19875, EYY34_13765, EYY78_14820, ExPECSC019_02707, ExPECSC038_04651, ExPECSC065_03193, F1E13_09345, F1E19_03375, F7F00_14880, F7F11_04220, F7F18_10000, F7F23_25375, F7F26_26295, F7F29_01940, F7G01_00535, F7G03_07175, F9059_10080, F9Z74_02985, FAF34_003085, FE846_00125, FKO60_18130, FNJ67_24100, FNJ69_19940, FNJ83_25610, FORC82_4346, FQ022_13705, FQ915_09820, FQR64_21020, FQU83_24305, FQZ46_22770, FTV92_15760, FV293_00695, FV295_12225, FV438_02410, FVB16_06060, FY127_22265, FZ043_01940, GFU40_14935, GFU45_12425, GFU47_18795, GII67_18045, GII91_15055, GIY19_14585, GJ11_26030, GJ638_00565, GJD97_25060, GKE15_09275, GKE22_10295, GKE24_11420, GKE26_10775, GKE29_08310, GKE31_09560, GKE39_09155, GKE46_08955, GKE58_08785, GKE60_08855, GKE64_08830, GKE77_10075, GKE87_04060, GKE92_06735, GKE93_00650, GKF03_06795, GKF28_04865, GKF34_05660, GKF47_05655, GKF74_12750, GKF86_05330, GKF89_03295, GKG08_03500, GKG09_04295, GKG11_04295, GKG22_04295, GKG29_04295, GN312_11965, GNZ00_20445, GNZ02_08740, GNZ03_05040, GP654_20575, GP661_16060, GP664_24795, GP666_16195, GP689_08440, GP700_05565, GP712_07845, GP720_11720, GP727_03900, GP912_07820, GP935_18020, GP946_12790, GQE22_08265, GQE30_14340, GQE34_01865, GQE42_10270, GQE47_08935, GQE51_14690, GQE58_15895, GQE64_09275, GQE68_13620, GQE88_08730, GQE93_06555, GQL64_02350, GQM09_07865, GQM13_10590, GQM17_01930, GQN16_09890, GQN33_18275, GQS26_16025, GRW42_14445, GRW80_01165, HMPREF3040_03032, HmCms169_03328, HmCms184_03267, HmCmsJML074_01333, HmCmsJML079_00812, HmCmsJML146_00635, HmCmsJML204_04031, MJ49_08265, MS6198_48630, MS8345_04771, NCTC10082_01857, NCTC10089_04560, NCTC10090_02607, NCTC10418_06721, NCTC10764_04513, NCTC10767_00467, NCTC10865_05650, NCTC10963_04614, NCTC10974_05136, NCTC11022_04473, NCTC11112_02719, NCTC11181_01789, NCTC11341_03019, NCTC12650_04817, NCTC12950_04949, NCTC13127_05944, NCTC13216_02274, NCTC13846_04307, NCTC7927_05027, NCTC8450_00576, NCTC8500_05095, NCTC8621_04680, NCTC8622_02021, NCTC8959_04316, NCTC8960_02051, NCTC9007_00921, NCTC9036_04433, NCTC9044_02622, NCTC9045_05354, NCTC9050_02551, NCTC9055_01424, NCTC9058_02377, NCTC9062_03620, NCTC9073_03984, NCTC9077_05690, NCTC9081_01494, NCTC9111_04720, NCTC9117_05641, NCTC9119_04735, NCTC9434_02173, NCTC9701_04822, NCTC9702_05313, NCTC9703_03911, NCTC9706_01822, NCTC9777_00950, NCTC9969_04590, PGD_03325, PU06_23700, RG28_06025, RK56_012245, RX35_04405, SAMEA3472043_02122, SAMEA3472044_01554, SAMEA3472047_01720, SAMEA3472055_04142, SAMEA3472056_01662, SAMEA3472070_02799, SAMEA3472080_00128, SAMEA3472090_01020, SAMEA3472110_00754, SAMEA3472112_01087, SAMEA3472114_01908, SAMEA3484427_02051, SAMEA3484429_03091, SAMEA3484434_04204, SAMEA3485101_04769, SAMEA3485113_03474, SAMEA3752372_01184, SAMEA3752553_04400, SAMEA3752559_00182, SAMEA3752620_03454, SAMEA3753064_01580, SAMEA3753097_01210, SAMEA3753164_04038, SAMEA3753290_02069, SAMEA3753300_00682, SK85_04506, SY51_23895, U12A_04548, U14A_04542, UC41_03555, UN91_24955, WQ89_21775, WR15_07175, YDC107_2866, groEL, groL, groL_1, groL_2, mopA
Sequence domains: TCP-1/cpn60 chaperonin family
Structure domains:

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P212121
Unit cell:
a: 136.24Å b: 261.83Å c: 282.28Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.198 0.223