4hbt

X-ray diffraction
1.1Å resolution

Crystal structure of native CTX-M-15 extended-spectrum beta-lactamase

Released:

Function and Biology Details

Reaction catalysed:
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactamase Chain: A
Molecule details ›
Chain: A
Length: 263 amino acids
Theoretical weight: 28.14 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9EXV5 (Residues: 29-291; Coverage: 100%)
Gene names: A9P13_28235, A9X72_26855, ACN68_29740, BANRA_05422, BFD68_26405, C6B13_05835, CCZ14_27365, CCZ17_27530, CIG45_26350, CO706_01125, CRM83_00400, CTX-M-15, D3O91_27855, D4718_22360, D4718_26245, D6T60_27795, DBQ99_00070, DM280_13930, DM280_26380, DMZ50_27430, DMZ50_28315, E0K84_20375, E0L04_26345, E0L12_27425, E2148_20410, E5P22_18740, EA191_28785, EA435_19100, EA834_25795, ECONIH1_02760, ECONIH1_27135, ECTO124_05284, EGT48_26805, ELT22_08720, ELT48_21595, ELT48_27155, ELT49_23685, ELU96_05460, ELV13_26970, ELX56_25110, ELX56_26475, ELX70_23835, ELX76_26235, ELX76_27770, ELX79_26345, ELX83_26200, ELX83_26525, ELY24_26875, EQ820_22590, EQ830_27280, ERL57_00245, EVY14_26065, EXX13_27820, EXX53_26085, ExPECSC065_05080, F9B07_28185, FNJ67_26095, G3565_28475, G5603_26845, G5616_24525, G5632_10280, GJ638_24945, GQE22_24020, GQE30_04350, GQE33_00910, GQE34_27530, GQE42_24520, GQE51_13305, GQE64_24895, GQE87_24455, GQE88_23895, GQE93_23330, GQL64_22180, GQS26_25095, GQY14_26540, GRW77_24730, GZS12_01600, MS6198_B109, RCS105_pI0076, RCS46_P0095, RCS50_P0027, RCS53_P0006, RCS57_p0080, RCS59_P0138, RCS67_P0011, SAMEA4363221_00081, SAMEA4370330_00042, SAMEA4370365_00031, SAMEA4370386_00071, SAMEA4370473_00021, bla, bla CTX-M-15, bla CTXM-15, blaCTX-M, blaCTX-M-15, blaCTX-M15, blaUOE-1, bla_1, bla_2, bla_3
Sequence domains: Beta-lactamase enzyme family
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P212121
Unit cell:
a: 44.698Å b: 45.72Å c: 117.086Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.118 0.117 0.136
Expression system: Escherichia coli