› 4hb9

X-ray diffraction
1.93Å resolution

Crystal structure of a putative FAD containing monooxygenase from Photorhabdus luminescens subsp. laumondii TTO1 (Target PSI-012791)

Released:
DOI: 10.2210/pdb4hb9/pdb
PDB entry 4hb9 coloured by chain, front view.
PDB entry 4hb9 coloured by chain, side view.
PDB entry 4hb9 coloured by chain, top view.
Source organism: Photorhabdus laumondii subsp. laumondii TTO1
Primary publication:
Stilbene epoxidation and detoxification in a Photorhabdus luminescens-nematode symbiosis.
Park HB, Sampathkumar P, Perez CE, Lee JH, Tran J, Bonanno JB, Hallem EA, Almo SC, Crawford JM
J Biol Chem 292 6680-6694 (2017)
PMID: 28246174

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181796 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
FAD-binding domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 412 amino acids
Theoretical weight: 46.26 KDa
Source organism: Photorhabdus laumondii subsp. laumondii TTO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7N4T1 (Residues: 2-411; Coverage: 100%)
Gene name: plu2236
Sequence domains:
Structure domains: FAD/NAD(P)-binding domain

Ligands and Environments

2 bound ligands:
Ligand FAD 1 x FAD
Ligand EDO 10 x EDO
1 modified residue:
Ligand MSE 11 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P3221
Unit cell:
a: 71.008Å b: 71.008Å c: 157.91Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.177 0.175 0.218
Expression system: Escherichia coli BL21(DE3)

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Citations

5 review citations

Chemical language and warfare of bacterial natural products in bacteria-nematode-insect interactions.
Shi et al. (2018)
4 more

3 mentions without citation

Deletion and gene expression analyses define the paxilline biosynthetic gene cluster in Penicillium paxilli.
Scott et al. (2013)
2 more