4h9d

X-ray diffraction
2.6Å resolution

Crystal Structure of Mn-dependent Gme HNH nicking endonuclease from Geobacter metallireducens GS-15, Northeast Structural Genomics Consortium (NESG) Target GmR87

Released:
Entry authors: Kuzin A, Chen Y, Seetharaman J, Fang F, Xiao R, Cunningham K, Ma L, Owens L, Chen CX, Everett JK, Acton TB, Montelione GT, Hunt JF, Tong L, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Biochemical function:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HNHc domain-containing protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 112 amino acids
Theoretical weight: 13.38 KDa
Source organism: Geobacter metallireducens GS-15
UniProt:
  • Canonical: Q39X46 (Residues: 1-104; Coverage: 100%)
Gene name: Gmet_0936
Sequence domains: HNH endonuclease
Structure domains: Herpes Virus-1

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: I222
Unit cell:
a: 40.674Å b: 83.385Å c: 225.116Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.233 0.251