› 4h4y

X-ray diffraction
1.9Å resolution

Crystal Structure of Ferredoxin reductase, BphA4 E175A/T176R/Q177G mutant (reduced form)

Released:
DOI: 10.2210/pdb4h4y/pdb
PDB entry 4h4y coloured by chain, front view.
PDB entry 4h4y coloured by chain, side view.
PDB entry 4h4y coloured by chain, top view.
Source organism: Acidovorax sp. KKS102
Entry authors: Nishizawa A, Harada A, Senda M, Tachihara Y, Muramatsu D, Kishigami S, Mori S, Sugiyama K, Senda T, Kimura S

Function and Biology Details

Reaction catalysed: 
2 reduced [2Fe-2S] ferredoxin + NAD(+) + H(+) = 2 oxidized [2Fe-2S] ferredoxin + NADH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123095 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Biphenyl dioxygenase ferredoxin reductase subunit Chain: A
Molecule details ›
Chain: A
Length: 408 amino acids
Theoretical weight: 43.15 KDa
Source organism: Acidovorax sp. KKS102
Expression system: Escherichia coli
UniProt:
  • Canonical: E7FJB9 (Residues: 1-408; Coverage: 100%)
Gene name: bphA4
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD

Cofactor: Ligand NAP 1 x NAP
2 bound ligands:
Ligand GOL 1 x GOL
Ligand FMT 8 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P6122
Unit cell:
a: 97.934Å b: 97.934Å c: 171.733Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.193 0.191 0.228
Expression system: Escherichia coli

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