4h3t

X-ray diffraction
2.03Å resolution

Crystal structure of CRISPR-associated protein Cse1 from Acidimicrobium ferrooxidans

Released:
Entry authors: Michalska K, Stols L, Clancy S, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CRISPR-associated protein, Cse1 family Chain: A
Molecule details ›
Chain: A
Length: 543 amino acids
Theoretical weight: 60.41 KDa
Source organism: Acidimicrobium ferrooxidans DSM 10331
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C7LYW9 (Residues: 1-540; Coverage: 100%)
Gene name: Afer_0988
Sequence domains: CRISPR-associated protein Cse1 (CRISPR_cse1)
Structure domains: Topoisomerase I; Chain A, domain 4

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: I4122
Unit cell:
a: 137.017Å b: 137.017Å c: 203.537Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.182 0.199
Expression system: Escherichia coli BL21(DE3)