4h3h

X-ray diffraction
2.2Å resolution

Crystal structure of a ternary complex of human symplekin NTD, human Ssu72 and a RNA poymerase II CTD peptide phosphorylated at SER-7

Released:

Function and Biology Details

Reaction catalysed:
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate

Structure analysis Details

Assemblies composition:
hetero dimer
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Symplekin Chains: A, D
Molecule details ›
Chains: A, D
Length: 351 amino acids
Theoretical weight: 39.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q92797 (Residues: 30-360; Coverage: 26%)
Gene names: SPK, SYMPK
Sequence domains: Domain of unknown function (DUF3453)
Structure domains: Leucine-rich Repeat Variant
RNA polymerase II subunit A C-terminal domain phosphatase SSU72 Chains: B, E
Molecule details ›
Chains: B, E
Length: 214 amino acids
Theoretical weight: 24.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NP77 (Residues: 1-194; Coverage: 100%)
Gene names: HSPC182, PNAS-120, SSU72
Sequence domains: Ssu72-like protein
Structure domains: Rossmann fold
Pol II CTD peptide Chain: F
Molecule details ›
Chain: F
Length: 10 amino acids
Theoretical weight: 1.1 KDa

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 66.819Å b: 97.742Å c: 104.603Å
α: 90° β: 98.63° γ: 90°
R-values:
R R work R free
0.199 0.198 0.227
Expression system: Escherichia coli