4h2h

X-ray diffraction
1.7Å resolution

Crystal structure of an enolase (mandalate racemase subgroup, target EFI-502101) from Pelagibaca bermudensis htcc2601, with bound mg and l-4-hydroxyproline betaine (betonicine)

Released:
Entry authors: Vetting MW, Morisco LL, Wasserman SR, Sojitra S, Imker HJ, Gerlt JA, Almo SC, Enzyme Function Initiative (EFI)

Function and Biology Details

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4-hydroxyproline betaine 2-epimerase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 376 amino acids
Theoretical weight: 40.7 KDa
Source organism: Salipiger bermudensis HTCC2601
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q0FPQ4 (Residues: 2-367; Coverage: 100%)
Gene names: R2601_01638, hpbD
Sequence domains:
Structure domains:

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P21
Unit cell:
a: 91.035Å b: 152.824Å c: 113.027Å
α: 90° β: 105.2° γ: 90°
R-values:
R R work R free
0.16 0.159 0.192
Expression system: Escherichia coli BL21(DE3)