› 4h2g

X-ray diffraction
1.55Å resolution

Human ecto-5'-nucleotidase (CD73): crystal form II (open) in complex with adenosine

Released:
DOI: 10.2210/pdb4h2g/pdb
PDB entry 4h2g coloured by chain, front view.
PDB entry 4h2g coloured by chain, side view.
PDB entry 4h2g coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the human ecto-5'-nucleotidase (CD73): insights into the regulation of purinergic signaling.
Knapp K, Zebisch M, Pippel J, El-Tayeb A, Müller CE, Sträter N
Structure 20 2161-73 (2012)
PMID: 23142347

Function and Biology Details

Reactions catalysed: 
Thymidylate + H(2)O = thymidine + phosphate
A 5'-ribonucleotide + H(2)O = a ribonucleoside + phosphate
A 2'-deoxyribonucleoside 5'-monophosphate + H(2)O = a 2'-deoxyribonucleoside + phosphate
CMP + H(2)O = cytidine + phosphate
IMP + H(2)O = inosine + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149316 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
5'-nucleotidase Chain: A
Molecule details ›
Chain: A
Length: 546 amino acids
Theoretical weight: 60.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P21589 (Residues: 27-549; Coverage: 95%)
Gene names: NT5, NT5E, NTE
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand ZN 2 x ZN
Ligand ADN 1 x ADN
Ligand CA 1 x CA
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P21212
Unit cell:
a: 67.1Å b: 131Å c: 66.01Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.129 0.129 0.18
Expression system: Escherichia coli

Quick links

Citations

31 review citations

CD39 and CD73 in immunity and inflammation.
Antonioli et al. (2013)
30 more

12 mentions without citation

Survey of ribose ring pucker of signaling nucleosides and nucleotides.
Salmaso et al. (2020)
11 more