› 4gz6

X-ray diffraction
2.98Å resolution

Crystal structure of human O-GlcNAc Transferase with UDP-5SGlcNAc

Released:
DOI: 10.2210/pdb4gz6/pdb
PDB 4gz6 coloured by chain and viewed from the front.
PDB 4gz6 coloured by chain and viewed from the side.
PDB 4gz6 coloured by chain and viewed from the top.
Source organism: Homo sapiens
Primary publication:
Structural snapshots of the reaction coordinate for O-GlcNAc transferase.
Lazarus MB, Jiang J, Gloster TM, Zandberg WF, Whitworth GE, Vocadlo DJ, Walker S
Nat. Chem. Biol. 8 966-8 (2012)
PMID: 23103939

Function and Biology Details

Reaction catalysed: 
UDP-N-acetyl-alpha-D-glucosamine + [protein]-L-serine = UDP + [protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dodecamer
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 723 amino acids
Theoretical weight: 80.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O15294 (Residues: 323-1041; Coverage: 69%)
Gene name: OGT
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand 12V 4 x 12V
Ligand SO4 17 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P321
Unit cell:
a: 272.338Å b: 272.338Å c: 143.46Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.198 0.197 0.223
Expression system: Escherichia coli

Quick links

Citations

19 review citations

A perspective on the PDB's impact on the field of glycobiology.
Prestegard JH. (2021)
18 more

1 mention without citation

Structural snapshots of the reaction coordinate for O-GlcNAc transferase.
Lazarus et al. (2012)