4gyp

X-ray diffraction
2.1Å resolution

Crystal structure of the heterotetrameric complex of GlucD and GlucDRP from E. coli K-12 MG1655 (EFI TARGET EFI-506058)

Released:
Source organism: Escherichia coli K-12
Entry authors: Lukk T, Ghasempur S, Imker HJ, Gerlt JA, Nair SK, Enzyme Function Initiative (EFI)

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Glucarate dehydratase Chains: A, B
Molecule details ›
Chains: A, B
Length: 446 amino acids
Theoretical weight: 49.2 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AES2 (Residues: 1-446; Coverage: 100%)
Gene names: JW2758, b2787, gudD, ygcX
Sequence domains: Enolase C-terminal domain-like
Structure domains:
Glucarate dehydratase-related protein Chains: C, D
Molecule details ›
Chains: C, D
Length: 458 amino acids
Theoretical weight: 50.4 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q46915 (Residues: 2-446; Coverage: 100%)
Gene names: JW2759, b2788, gudX, ygcY
Sequence domains:
Structure domains:

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: C2
Unit cell:
a: 155.47Å b: 113.02Å c: 128.59Å
α: 90° β: 105.89° γ: 90°
R-values:
R R work R free
0.172 0.149 0.186
Expression system: Escherichia coli BL21(DE3)