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Distinct gating mechanisms revealed by the structures of a multi-ligand gated K(+) channel.

Kong C, Zeng W, Ye S, Chen L, Sauer DB, Lam Y, Derebe MG, Jiang Y
Elife 1 e00184 (2012)
Related entries: 4gvl, 4gx1, 4gx2, 4gx5

Cited: 19 times
EuropePMC logo PMID: 23240087

Abstract

The gating ring-forming RCK domain regulates channel gating in response to various cellular chemical stimuli in eukaryotic Slo channel families and the majority of ligand-gated prokaryotic K(+) channels and transporters. Here we present structural and functional studies of a dual RCK-containing, multi-ligand gated K(+) channel from Geobacter sulfurreducens, named GsuK. We demonstrate that ADP and NAD(+) activate the GsuK channel, whereas Ca(2+) serves as an allosteric inhibitor. Multiple crystal structures elucidate the structural basis of multi-ligand gating in GsuK, and also reveal a unique ion conduction pore with segmented inner helices. Structural comparison leads us to propose a novel pore opening mechanics that is distinct from other K(+) channels.DOI:http://dx.doi.org/10.7554/eLife.00184.001.

Articles - 4gx0 mentioned but not cited (2)

  1. Distinct gating mechanisms revealed by the structures of a multi-ligand gated K(+) channel. Kong C, Zeng W, Ye S, Chen L, Sauer DB, Lam Y, Derebe MG, Jiang Y. Elife 1 e00184 (2012)
  2. Computational Analysis of the Crystal and Cryo-EM Structures of P-Loop Channels with Drugs. Tikhonov DB, Zhorov BS. Int J Mol Sci 22 8143 (2021)


Reviews citing this publication (7)

  1. BK channels: multiple sensors, one activation gate. Yang H, Zhang G, Cui J. Front Physiol 6 29 (2015)
  2. Molecular Mechanisms for Bacterial Potassium Homeostasis. Stautz J, Hellmich Y, Fuss MF, Silberberg JM, Devlin JR, Stockbridge RB, Hänelt I. J Mol Biol 433 166968 (2021)
  3. The evolution of bacterial mechanosensitive channels. Booth IR, Miller S, Müller A, Lehtovirta-Morley L. Cell Calcium 57 140-150 (2015)
  4. Recent progress on the structure and function of the TrkH/KtrB ion channel. Levin EJ, Zhou M. Curr Opin Struct Biol 27 95-101 (2014)
  5. Understanding the conformational motions of RCK gating rings. Giraldez T, Rothberg BS. J Gen Physiol 149 431-441 (2017)
  6. Abundant Monovalent Ions as Environmental Signposts for Pathogens during Host Colonization. Tan S. Infect Immun 89 e00641-20 (2021)
  7. P-Loop Channels: Experimental Structures, and Physics-Based and Neural Networks-Based Models. Tikhonov DB, Zhorov BS. Membranes (Basel) 12 229 (2022)

Articles citing this publication (10)

  1. Gating of the TrkH ion channel by its associated RCK protein TrkA. Cao Y, Pan Y, Huang H, Jin X, Levin EJ, Kloss B, Zhou M. Nature 496 317-322 (2013)
  2. Cryo-EM structure of the lysosomal chloride-proton exchanger CLC-7 in complex with OSTM1. Schrecker M, Korobenko J, Hite RK. Elife 9 e59555 (2020)
  3. Mutation of a single residue promotes gating of vertebrate and invertebrate two-pore domain potassium channels. Ben Soussia I, El Mouridi S, Kang D, Leclercq-Blondel A, Khoubza L, Tardy P, Zariohi N, Gendrel M, Lesage F, Kim EJ, Bichet D, Andrini O, Boulin T. Nat Commun 10 787 (2019)
  4. Ca2+-regulated Ca2+ channels with an RCK gating ring control plant symbiotic associations. Kim S, Kim S, Zeng W, Bernard S, Liao J, Venkateshwaran M, Ane JM, Jiang Y. Nat Commun 10 3703 (2019)
  5. Structural basis of allosteric interactions among Ca2+-binding sites in a K+ channel RCK domain. Smith FJ, Pau VP, Cingolani G, Rothberg BS. Nat Commun 4 2621 (2013)
  6. Dissecting the Molecular Mechanism of Nucleotide-Dependent Activation of the KtrAB K+ Transporter. Szollosi A, Vieira-Pires RS, Teixeira-Duarte CM, Rocha R, Morais-Cabral JH. PLoS Biol 14 e1002356 (2016)
  7. Helical jackknives control the gates of the double-pore K+ uptake system KtrAB. Diskowski M, Mehdipour AR, Wunnicke D, Mills DJ, Mikusevic V, Bärland N, Hoffmann J, Morgner N, Steinhoff HJ, Hummer G, Vonck J, Hänelt I. Elife 6 e24303 (2017)
  8. Adenosine Monophosphate Binding Stabilizes the KTN Domain of the Shewanella denitrificans Kef Potassium Efflux System. Pliotas C, Grayer SC, Ekkerman S, Chan AKN, Healy J, Marius P, Bartlett W, Khan A, Cortopassi WA, Chandler SA, Rasmussen T, Benesch JLP, Paton RS, Claridge TDW, Miller S, Booth IR, Naismith JH, Conway SJ. Biochemistry 56 4219-4234 (2017)
  9. Activation of a nucleotide-dependent RCK domain requires binding of a cation cofactor to a conserved site. Teixeira-Duarte CM, Fonseca F, Morais-Cabral JH. Elife 8 e50661 (2019)
  10. Crystal structure of a two-subunit TrkA octameric gating ring assembly. Deller MC, Johnson HA, Miller MD, Spraggon G, Elsliger MA, Wilson IA, Lesley SA. PLoS One 10 e0122512 (2015)


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