PDBe 4gqb

X-ray diffraction
2.06Å resolution

Crystal Structure of the human PRMT5:MEP50 Complex


Function and Biology Details

Reaction catalysed:
(1a) S-adenosyl-L-methionine + [protein]-L-arginine = S-adenosyl-L-homocysteine + [protein]-N(omega)-methyl-L-arginine
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero 12-mer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Protein arginine N-methyltransferase 5 Chain: A
Molecule details ›
Chain: A
Length: 637 amino acids
Theoretical weight: 72.77 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
  • Canonical: O14744 (Residues: 1-637; Coverage: 100%)
Gene names: HRMT1L5, IBP72, JBP1, PRMT5, SKB1
Sequence domains:
Structure domains:
Methylosome protein 50 Chain: B
Molecule details ›
Chain: B
Length: 344 amino acids
Theoretical weight: 36.88 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
  • Canonical: Q9BQA1 (Residues: 2-342; Coverage: 100%)
Gene names: HKMT1069, MEP50, Nbla10071, WD45, WDR77
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
Histone H4 Chain: C
Molecule details ›
Chain: C
Length: 22 amino acids
Theoretical weight: 2.13 KDa
Source organism: Homo sapiens
Expression system: Not provided
  • Canonical: P62805 (Residues: 2-22; Coverage: 20%)
Gene names: H4/A, H4/B, H4/C, H4/D, H4/E, H4/G, H4/H, H4/I, H4/J, H4/K, H4/M, H4/N, H4/O, H4F2, H4FA, H4FB, H4FC, H4FD, H4FE, H4FG, H4FH, H4FI, H4FJ, H4FK, H4FM, H4FN, H4FO, HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4K, HIST1H4L, HIST2H4, HIST2H4A, HIST2H4B, HIST4H4

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: I222
Unit cell:
a: 101.981Å b: 138.762Å c: 178.472Å
α: 90° β: 90° γ: 90°
R R work R free
0.19 0.188 0.222
Expression systems:
  • Spodoptera frugiperda
  • Not provided