4gon

X-ray diffraction
2.72Å resolution

Crystal Structure of E. coli DNA Adenine Methyltransferase in Complex with Indole Aza-SAM

Released:
Source organism: Escherichia coli K-12
Entry authors: Harmer JE, Roach PL

Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + adenine in DNA = S-adenosyl-L-homocysteine + N-6-methyladenine in DNA 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
homo trimer
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA adenine methylase Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 278 amino acids
Theoretical weight: 32.14 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AEE8 (Residues: 1-278; Coverage: 100%)
Gene names: JW3350, b3387, dam
Sequence domains: D12 class N6 adenine-specific DNA methyltransferase
Structure domains:

Ligands and Environments


Cofactor: Ligand 0Y1 3 x 0Y1
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P3121
Unit cell:
a: 161.315Å b: 161.315Å c: 95.134Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.212 0.21 0.258
Expression system: Escherichia coli BL21(DE3)