PDBe 4gmj

X-ray diffraction
2.7Å resolution

Structure of human NOT1 MIF4G domain co-crystallized with CAF1

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
CCR4-NOT transcription complex subunit 1 Chains: A, C, E
Molecule details ›
Chains: A, C, E
Length: 229 amino acids
Theoretical weight: 26.63 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: A5YKK6 (Residues: 1093-1317; Coverage: 10%)
Gene names: AD-005, CDC39, CNOT1, KIAA1007, NOT1
Structure domains: Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat
CCR4-NOT transcription complex subunit 7 Chains: B, D, F
Molecule details ›
Chains: B, D, F
Length: 285 amino acids
Theoretical weight: 32.78 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UIV1 (Residues: 1-285; Coverage: 100%)
Gene names: CAF1, CNOT7
Sequence domains: CAF1 family ribonuclease
Structure domains: Ribonuclease H-like superfamily/Ribonuclease H

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21
Unit cell:
a: 82.47Å b: 101.74Å c: 142.2Å
α: 90° β: 101.1° γ: 90°
R-values:
R R work R free
0.215 0.214 0.249
Expression system: Escherichia coli