› 4gmf

X-ray diffraction
1.85Å resolution

Apo Structure of a Thiazolinyl Imine Reductase from Yersinia enterocolitica (Irp3)

Released:
DOI: 10.2210/pdb4gmf/pdb
PDB entry 4gmf coloured by chain, front view.
PDB entry 4gmf coloured by chain, side view.
PDB entry 4gmf coloured by chain, top view.
Source organism: Yersinia enterocolitica subsp. enterocolitica 8081
Primary publication:
Two structures of a thiazolinyl imine reductase from Yersinia enterocolitica provide insight into catalysis and binding to the nonribosomal peptide synthetase module of HMWP1.
Meneely KM, Lamb AL
Biochemistry 51 9002-13 (2012)
PMID: 23066849

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106636 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Yersiniabactin biosynthetic protein YbtU Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 372 amino acids
Theoretical weight: 41.71 KDa
Source organism: Yersinia enterocolitica subsp. enterocolitica 8081
Expression system: Escherichia coli
UniProt:
  • Canonical: A1JTG0 (Residues: 2-366; Coverage: 100%)
Gene names: YE2619, irp3, ybtU
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand SO4 4 x SO4
Ligand EDO 2 x EDO
Ligand EPE 1 x EPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P212121
Unit cell:
a: 83.871Å b: 93.903Å c: 181.121Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.196 0.242
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Imine reductases (IREDs).
Mangas-Sanchez et al. (2017)
1 more

2 mentions without citation

Two structures of a thiazolinyl imine reductase from Yersinia enterocolitica provide insight into catalysis and binding to the nonribosomal peptide synthetase module of HMWP1.
Meneely et al. (2012)
1 more