› 4gkx

X-ray diffraction
2.7Å resolution

Crystal structure of a carbohydrate-binding domain

Released:
DOI: 10.2210/pdb4gkx/pdb
PDB entry 4gkx coloured by chain, front view.
PDB entry 4gkx coloured by chain, side view.
PDB entry 4gkx coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural characterization of carbohydrate binding by LMAN1 protein provides new insight into the endoplasmic reticulum export of factors V (FV) and VIII (FVIII).
Zheng C, Page RC, Das V, Nix JC, Wigren E, Misra S, Zhang B
J Biol Chem 288 20499-509 (2013)
PMID: 23709226

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155903 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Protein ERGIC-53 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 261 amino acids
Theoretical weight: 28.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P49257 (Residues: 31-270; Coverage: 50%)
Gene names: ERGIC53, F5F8D, LMAN1
Sequence domains: Legume-like lectin family
Structure domains: Jelly Rolls

Ligands and Environments

Carbohydrate polymer : NEW Components: MAN
Carbohydrate polymer
2 bound ligands:
Ligand CA 12 x CA
Ligand GOL 7 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P1
Unit cell:
a: 39.283Å b: 111.498Å c: 111.388Å
α: 60.09° β: 89.21° γ: 86.25°
R-values:
R R work R free
0.248 0.246 0.286
Expression system: Escherichia coli BL21

Quick links

Citations

9 review citations

Convergent and divergent mechanisms of sugar recognition across kingdoms.
Taylor et al. (2014)
8 more

5 mentions without citation

Insights into animal and plant lectins with antimicrobial activities.
Dias Rde et al. (2015)
4 more