4gho

X-ray diffraction
1.1Å resolution

Crystal Structure Analysis of Streptomyces aureofaciens Ribonuclease S24A mutant

Released:

Function and Biology Details

Reaction catalysed:
(1a) (RNA) containing guanosine = (RNA fragment)-3'-guanosine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA fragment)
Biochemical function:
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
PDBe Complex ID:
PDB-CPX-138780 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Guanyl-specific ribonuclease Sa Chains: A, B
Molecule details ›
Chains: A, B
Length: 96 amino acids
Theoretical weight: 10.57 KDa
Source organism: Kitasatospora aureofaciens
Expression system: Escherichia coli
UniProt:
  • Canonical: P05798 (Residues: 1-96; Coverage: 100%)
Gene name: rnaSA
Sequence domains: ribonuclease
Structure domains: Microbial ribonucleases

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: P212121
Unit cell:
a: 38.918Å b: 64.693Å c: 78.281Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.098 0.097 0.117
Expression system: Escherichia coli