› 4ggv

X-ray diffraction
2.33Å resolution

Crystal Structure of HmtT Involved in Himastatin Biosynthesis

Released:
DOI: 10.2210/pdb4ggv/pdb
PDB entry 4ggv coloured by chain, front view.
PDB entry 4ggv coloured by chain, side view.
PDB entry 4ggv coloured by chain, top view.
Source organism: Streptomyces himastatinicus ATCC 53653
Primary publication:
Structural analysis of HmtT and HmtN involved in the tailoring steps of himastatin biosynthesis.
Zhang H, Chen J, Wang H, Xie Y, Ju J, Yan Y, Zhang H
FEBS Lett 587 1675-80 (2013)
PMID: 23611984

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113037 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative P450-like enzyme Chain: A
Molecule details ›
Chain: A
Length: 418 amino acids
Theoretical weight: 46.48 KDa
Source organism: Streptomyces himastatinicus ATCC 53653
Expression system: Escherichia coli
UniProt:
  • Canonical: D9WMR2 (Residues: 1-397; Coverage: 100%)
Gene names: SSOG_07642, hmtT
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments

1 bound ligand:
Ligand HEM 1 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21212
Unit cell:
a: 64.41Å b: 121.139Å c: 54.731Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.208 0.257
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Unrivalled diversity: the many roles and reactions of bacterial cytochromes P450 in secondary metabolism.
Greule et al. (2018)
4 more

4 mentions without citation

Cytochromes P450 for natural product biosynthesis in Streptomyces: sequence, structure, and function.
Rudolf et al. (2017)
3 more