PDBe 4ggd

X-ray diffraction
2.44Å resolution

Structural analysis of human Cdc20 supports multisite degron recognition by APC/C.

Released:
Source organism: Homo sapiens
Primary publication:
Structural analysis of human Cdc20 supports multisite degron recognition by APC/C.
Proc. Natl. Acad. Sci. U.S.A. (2012)
PMID: 23091007

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cell division cycle protein 20 homolog Chains: A, B
Molecule details ›
Chains: A, B
Length: 431 amino acids
Theoretical weight: 47.5 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q12834 (Residues: 71-499; Coverage: 86%)
Gene name: CDC20
Sequence domains:
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
Mitotic checkpoint serine/threonine-protein kinase BUB1 beta Chains: C, D
Molecule details ›
Chains: C, D
Length: 23 amino acids
Theoretical weight: 2.76 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: O60566 (Residues: 20-42; Coverage: 2%)
Gene names: BUB1B, BUBR1, MAD3L, SSK1

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 49.826Å b: 88.129Å c: 118.351Å
α: 90° β: 99.66° γ: 90°
R-values:
R R work R free
0.156 0.154 0.201
Expression systems:
  • Spodoptera frugiperda
  • Not provided