4gek

X-ray diffraction
1.5Å resolution

Function and Biology Details

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carboxy-S-adenosyl-L-methionine synthase Chains: A, G
Molecule details ›
Chains: A, G
Length: 261 amino acids
Theoretical weight: 29.54 KDa
Source organism: Escherichia coli str. K-12 substr. MG1655
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C3T5M2 (Residues: 1-247; Coverage: 100%)
Gene names: A6581_07795, A6592_10075, A6V01_11875, A9819_11150, A9R57_15505, A9X72_10450, ACN002_1905, ACN68_23480, ACN81_03915, ACU90_24675, AJ318_10475, AM270_14180, AM464_00105, AM465_16155, AMK83_04590, AML35_08280, APU18_08760, APZ14_24765, ARC77_04260, AU473_20330, AUQ13_06915, AUS26_11025, AW106_08005, AWB10_11060, AWF59_019540, AWG78_023210, AZZ83_004137, B6V57_09900, B7C53_14820, B9N33_23025, B9T59_01445, BANRA_02464, BANRA_02526, BB545_25105, BFD68_21600, BHF03_01010, BHS81_11390, BHS87_10570, BIQ87_10635, BIZ41_07775, BJJ90_14635, BK292_17305, BK334_13220, BK373_12865, BMT49_12240, BMT91_06995, BN17_22881, BOH76_14120, BON65_16615, BON66_16445, BON69_06040, BON72_12025, BON75_09270, BON86_05985, BON91_17635, BON92_21685, BON94_10355, BON95_14120, BTQ04_08390, BTQ06_25345, BVL39_18455, BWI89_04605, BWP17_07390, BZL31_12015, BvCms2454_03029, BvCms28BK_00225, BvCms35BK_00229, BvCmsHHP019_02030, BvCmsKSNP073_03883, BvCmsKSNP081_03986, BvCmsKSP076_00589, BvCmsNSP006_03884, BvCmsOUP014_01768, BvCmsSIP024_00567, C2M16_13865, C4K41_00560, C4M78_08795, C5P01_01205, C5P44_16255, C6B13_06085, C7235_10440, C7B02_20675, C7B06_07865, C7B07_07895, C7B18_07900, C9160_10515, C9201_06130, C9306_09125, C9E25_11990, C9E67_11845, C9Z03_14125, C9Z28_13910, C9Z37_00455, C9Z43_23010, C9Z70_02810, C9Z78_06070, C9Z89_11035, CCZ14_15005, CCZ17_21115, CDC27_09560, CDL37_01265, CG692_08685, CI641_002060, CJU63_10775, CJU64_11080, CO706_06770, COD30_05475, COD46_09430, CQP61_16690, CRD98_08405, CRE06_13480, CRM83_03675, CRT43_10640, CSB64_21235, CT146_08115, CUB99_24290, CVH05_08305, CWS33_16845, D2184_05250, D3M98_04495, D3O91_12570, D3Y67_13295, D4011_08770, D4023_09135, D4074_09740, D4628_09000, D4636_12925, D4660_10250, D4718_05730, D4L91_07875, D4M06_07145, D4M76_11795, D4U49_14845, D4U85_01600, D5H35_19720, D5I97_19360, D6004_12240, D6C36_09810, D6D43_06345, D6T60_19780, D6T98_10050, D6X36_16825, D6X63_21640, D6X76_15135, D7K33_19000, D7K63_06495, D7K66_12350, D7W70_01595, D7Y10_06690, D7Z75_12160, D8Y65_08515, D9610_05740, D9C99_06190, D9D33_14920, D9D94_15245, D9E35_04615, D9E49_18530, D9E73_05205, D9F32_19925, D9F87_00475, D9G11_10180, D9G42_10590, D9G95_05050, D9H10_14115, D9H53_12735, D9H70_00725, D9H94_05090, D9I18_10205, D9I20_09980, D9I87_17900, D9J03_15540, D9J44_20960, D9J61_10860, D9K02_16160, D9K48_07735, D9K54_06460, D9L89_12870, D9L99_06600, D9S45_17075, D9X77_08435, D9X97_12210, D9Z28_02995, DAH26_07985, DAH30_06535, DB359_04505, DEN89_00330, DEO04_05980, DEO20_12685, DIV22_08010, DL326_00605, DL455_08595, DL479_00205, DL530_13090, DL705_12490, DL800_09700, DL979_00885, DLT82_07835, DLU50_14045, DLU82_00410, DLW88_26565, DLX40_06480, DLY41_04675, DLY44_04315, DM155_11990, DM267_21790, DM296_25285, DM820_13805, DM962_08475, DMC44_01540, DMI04_07155, DMI53_09075, DMO02_10370, DMY83_24335, DMZ30_00375, DMZ50_18045, DN627_05960, DN660_16590, DN700_14720, DN703_11750, DNB37_09465, DNC98_18705, DND79_13045, DNI21_24270, DNJ62_09920, DNK12_09215, DNR41_00880, DNW42_20980, DNX30_00535, DOE35_20905, DOS18_18525, DOT81_10705, DOU81_17860, DOY22_11120, DOY56_17430, DOY61_07465, DOY67_15185, DP258_03325, DQF36_09615, DQF57_00805, DQG35_10585, DQO13_13830, DQP61_16715, DRP48_04465, DRW19_12965, DS143_07485, DS721_07380, DS966_16580, DT034_09530, DTL43_12005, DTM10_11485, DTM45_01875, DU333_03740, DVB38_10715, DXT69_14560, DXX80_026815, E0I42_15115, E0L04_17765, E2112_07460, E2127_12960, E2128_10015, E2129_09120, E2134_16335, E2135_11115, E2148_07970, E2855_02412, E2863_02301, E5P24_06820, E5P28_07280, E5S38_00390, E5S42_12300, E5S56_00385, EA159_22125, EA164_18655, EA167_21560, EA184_03650, EA191_20785, EA198_01770, EA200_00875, EA203_22540, EA218_19300, EA231_04290, EA239_03765, EA242_16900, EA245_13795, EA250_04075, EA429_06260, EA434_11910, EA435_22555, EA834_22485, EAI46_20990, EAM59_10595, EAN70_15280, EAN77_26870, EAX79_12270, EB476_00850, EB509_01870, EB510_09580, EB515_12925, EB525_RS09895, EBA46_12070, EBJ06_14080, EBM08_12280, EBP16_02445, EC1094V2_1870, EC3234A_35c00760, EC382_13115, EC95NR1_00866, ECONIH1_10750, ECTO124_02178, ECs2580, ED225_15100, ED600_07090, ED607_06980, ED611_14935, ED903_09000, ED944_13370, EEA45_11535, EF173_06375, EG075_03830, EG599_15715, EG796_23375, EG808_08315, EGC26_03750, EGT48_04155, EGU87_08035, EH186_05380, EH412_07560, EHD63_11420, EHD79_16745, EHH55_07745, EHJ36_07350, EHJ66_14635, EHV81_07005, EHV90_02875, EHW09_11430, EHX09_08190, EI032_22310, EIA08_13685, EIA21_09780, EIZ93_04095, EJC75_08135, EKI52_26655, EL75_1755, EL79_1814, EL80_1843, ELT22_00610, ELT23_17080, ELT31_14570, ELT33_07535, ELT48_09765, ELT49_17255, ELU82_20205, ELU96_04250, ELV00_14905, ELV08_07995, ELV13_21985, ELV22_15010, ELV24_01980, ELX56_17970, ELX68_23160, ELX69_00745, ELX70_25495, ELX79_15680, ELX83_09950, ELY23_19715, ELY24_12175, ELY50_20755, EO241_10585, EPS76_23410, EPS91_11710, EQ825_08770, EQO00_11195, ERS085365_02201, ERS085366_01771, ERS085379_01362, ERS085383_03504, ERS085404_00103, ERS085406_02498, ERS085416_01165, ERS139211_01821, ERS150873_00898, EWK56_00565, EXM29_22655, EXX06_02750, EXX13_10415, EXX23_01070, EXX24_13370, EXX53_00945, EXX55_05435, EXX87_14385, EYX82_00660, EYY27_23955, ExPECSC038_01841, ExPECSC065_02566, F0L67_08110, F1E19_20105, F7D02_06825, F7D05_13550, F7D06_09885, F7F11_18965, F7F23_08895, F7F26_07325, F7F56_21220, F7G03_11480, F9040_06535, F9Z74_04885, FE846_20355, FNJ67_11550, FNW97_10590, FORC82_2023, FPI65_11400, FQ021_01960, FQ022_01115, FQ915_22695, FQU83_12655, FQZ46_13325, FV295_03155, FV438_01315, FZN30_16225, G3565_19815, G5603_12890, G5608_07135, G5668_07210, G5670_11175, G5675_12800, G5680_12395, G5686_24195, GFU40_00845, GFU45_09190, GFU47_05320, GII67_04695, GIY19_03280, GJ638_17120, GJD95_10865, GJD97_11070, GKE92_19405, GKE98_01750, GKG12_10470, GNZ03_18915, GNZ05_22780, GP650_18500, GP654_01755, GP661_22470, GP662_08235, GP664_00135, GP678_03040, GP698_11480, GP711_12630, GP945_06670, GP946_06070, GQA63_08585, GQE47_12555, GQE58_03715, GQE64_06385, GQE93_03160, GQF59_10705, GQM04_22285, GQM10_10875, GQM13_04200, GQM28_00395, GQN16_19180, GQN24_19175, GQN33_11975, GQS26_08400, GQY14_04100, GRW30_19580, GRW42_07820, GRW80_04475, GUB85_00920, GUB91_04650, GUB95_00920, GUC01_04485, GUC12_00920, GUI33_22860, HPE49_03210, HPE52_08140, HR075_02690, HV022_09435, HV098_11040, HV156_23665, HV159_01750, HV168_01195, HV303_03025, HVW04_01310, HVW93_12870, HVX75_10130, HVY01_09695, HVY77_11010, HVZ21_09995, HVZ53_10135, HW43_13260, HXS78_09900, HZT35_28750, HmCms169_02644, HmCmsJML079_02764, HmCmsJML146_00964, HmCmsJML204_03057, MJ49_21980, MS6198_21020, MS8345_01982, NCTC10418_03207, NCTC10764_01555, NCTC10767_03665, NCTC10865_02651, NCTC10963_02120, NCTC11341_06106, NCTC13127_02792, NCTC7922_01906, NCTC7927_02355, NCTC7928_06142, NCTC8009_04910, NCTC8179_04118, NCTC8450_02413, NCTC8960_04894, NCTC9001_02626, NCTC9036_02134, NCTC9044_04826, NCTC9050_00177, NCTC9055_03976, NCTC9081_00134, NCTC9111_02169, NCTC9119_02264, NCTC9434_04439, NCTC9701_02244, NCTC9702_02511, NCTC9703_01361, NCTC9706_04302, NCTC9777_04008, NCTC9962_04528, NCTC9969_02248, PU06_26665, RG28_12600, RX35_00662, SAMEA3472044_04443, SAMEA3472047_00081, SAMEA3472055_00624, SAMEA3472056_01359, SAMEA3472090_03465, SAMEA3472108_01246, SAMEA3472147_03951, SAMEA3485101_04994, SAMEA3752553_03058, SAMEA3752620_03794, SAMEA3753097_04256, SAMEA3753164_03476, SK85_02098, SY51_10380, TUM18780_17950, U14A_01957, UN86_11355, WP4S18E07_19420, WP5S18E08_20490, WQ89_15790, cmoA, yecO
Sequence domains: Methyltransferase domain
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21212
Unit cell:
a: 65.314Å b: 78.677Å c: 92.372Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.171 0.203
Expression system: Escherichia coli BL21(DE3)