› 4gbm

X-ray diffraction
1.62Å resolution

Sulfotransferase Domain from the Curacin Biosynthetic Pathway

Released:
DOI: 10.2210/pdb4gbm/pdb
PDB 4gbm coloured by chain and viewed from the front.
PDB 4gbm coloured by chain and viewed from the side.
PDB 4gbm coloured by chain and viewed from the top.
Source organism: Moorea producens
Primary publication:
Structural Basis of Functional Group Activation by Sulfotransferases in Complex Metabolic Pathways.
McCarthy JG, Eisman EB, Kulkarni S, Gerwick L, Gerwick WH, Wipf P, Sherman DH, Smith JL
ACS Chem. Biol. (2012)
PMID: 22991895

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carrier domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 323 amino acids
Theoretical weight: 36.41 KDa
Source organism: Moorea producens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: F4Y423 (Residues: 1598-1917; Coverage: 15%)
Gene name: LYNGBM3L_74580
Sequence domains: Sulfotransferase family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

5 bound ligands:
Ligand ZN 5 x ZN
Ligand A3P 1 x A3P
Ligand CL 9 x CL
Ligand SO4 1 x SO4
Ligand P6G 2 x P6G
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P212121
Unit cell:
a: 45.856Å b: 67.299Å c: 118.016Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.16 0.158 0.182
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Biogenetic Relationships of Bioactive Sponge Merotriterpenoids.
Smith TE. (2017)
5 more

2 mentions without citation

Structural basis of functional group activation by sulfotransferases in complex metabolic pathways.
McCarthy et al. (2012)
1 more