PDBe 4g5r

X-ray diffraction
3.48Å resolution

Structure of LGN GL4/Galphai3 complex

Source organisms:
Entry authors: Jia M, Li J, Zhu J, Wen W, Zhang M, Wang W

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Guanine nucleotide-binding protein G(i) subunit alpha Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 330 amino acids
Theoretical weight: 37.9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: P08754 (Residues: 25-354; Coverage: 93%)
Gene name: GNAI3
Structure domains:
G-protein-signaling modulator 2 Chains: E, F, G, Z
Molecule details ›
Chains: E, F, G, Z
Length: 25 amino acids
Theoretical weight: 3.1 KDa
Source organism: Mus musculus
Expression system: Not provided
  • Canonical: Q8VDU0 (Residues: 628-652; Coverage: 4%)
Gene names: Gpsm2, Lgn, Pins
Sequence domains: GoLoco motif

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P6122
Unit cell:
a: 209.55Å b: 209.55Å c: 237.211Å
α: 90° β: 90° γ: 120°
R R work R free
0.211 0.209 0.253
Expression systems:
  • Escherichia coli
  • Not provided