4g5d

X-ray diffraction
1.8Å resolution

X-ray crystal structure of Prostaglandin f synthase from Leishmania major Friedlin bound to NADPH

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
9,11-endoperoxide prostaglandin H2 reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 288 amino acids
Theoretical weight: 32.19 KDa
Source organism: Leishmania major strain Friedlin
Expression system: Escherichia coli
UniProt:
  • Canonical: P22045 (Residues: 1-284; Coverage: 100%)
Gene name: P100/11E
Sequence domains: Aldo/keto reductase family
Structure domains: NADP-dependent oxidoreductase domain

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: P21
Unit cell:
a: 94.66Å b: 34.59Å c: 106.95Å
α: 90° β: 102.98° γ: 90°
R-values:
R R work R free
0.174 0.172 0.218
Expression system: Escherichia coli