› 4g3d

X-ray diffraction
2.9Å resolution

Crystal structure of human NF-kappaB inducing kinase (NIK)

Released:
DOI: 10.2210/pdb4g3d/pdb
PDB entry 4g3d coloured by chain, front view.
PDB entry 4g3d coloured by chain, side view.
PDB entry 4g3d coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The crystal structure of the catalytic domain of the NF-κB inducing kinase reveals a narrow but flexible active site.
de Leon-Boenig G, Bowman KK, Feng JA, Crawford T, Everett C, Franke Y, Oh A, Stanley M, Staben ST, Starovasnik MA, Wallweber HJ, Wu J, Wu LC, Johnson AR, Hymowitz SG
Structure 20 1704-14 (2012)
PMID: 22921830

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189222 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mitogen-activated protein kinase kinase kinase 14 Chains: A, B, D, E
Molecule details ›
Chains: A, B, D, E
Length: 371 amino acids
Theoretical weight: 40.71 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q99558 (Residues: 308-673; Coverage: 39%)
Gene names: MAP3K14, NIK
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand MG 3 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P212121
Unit cell:
a: 45.746Å b: 145.851Å c: 230.818Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.201 0.266
Expression system: Spodoptera frugiperda

Quick links

Citations

11 review citations

The non-canonical NF-κB pathway in immunity and inflammation.
Sun SC. (2017)
10 more

9 mentions without citation

Pharmacological inhibition of NF-κB-inducing kinase (NIK) with small molecules for the treatment of human diseases.
Cheng et al. (2021)
8 more