› 4fwx

X-ray diffraction
1.9Å resolution

Aquoferric F33Y CuB myoglobin (F33Y L29H F43H sperm whale myoglobin)

Released:
DOI: 10.2210/pdb4fwx/pdb
PDB entry 4fwx coloured by chain, front view.
PDB entry 4fwx coloured by chain, side view.
PDB entry 4fwx coloured by chain, top view.
Source organism: Physeter catodon
Primary publication:
A designed functional metalloenzyme that reduces O2 to H2O with over one thousand turnovers.
Miner KD, Mukherjee A, Gao YG, Null EL, Petrik ID, Zhao X, Yeung N, Robinson H, Lu Y
Angew Chem Int Ed Engl 51 5589-92 (2012)
PMID: 22539151

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-135409 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Myoglobin Chain: A
Molecule details ›
Chain: A
Length: 153 amino acids
Theoretical weight: 17.27 KDa
Source organism: Physeter catodon
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P02185 (Residues: 2-154; Coverage: 99%)
Gene name: MB
Sequence domains: Globin
Structure domains: Globins

Ligands and Environments

1 bound ligand:
Ligand HEM 1 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X26C
Spacegroup: P212121
Unit cell:
a: 39.219Å b: 47.718Å c: 77.089Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.211 0.266
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

18 review citations

Oxygen Activation and Radical Transformations in Heme Proteins and Metalloporphyrins.
Huang et al. (2018)
17 more

11 mentions without citation

Synthetic Fe/Cu Complexes: Toward Understanding Heme-Copper Oxidase Structure and Function.
Adam et al. (2018)
10 more