4fs2

X-ray diffraction
2.05Å resolution

Base pairing mechanism of N2,3-ethenoguanine with dCTP by human polymerase iota

Released:

Function and Biology Details

Reaction catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
DNA polymerase iota Chain: A
Molecule details ›
Chain: A
Length: 420 amino acids
Theoretical weight: 47.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UNA4 (Residues: 26-445; Coverage: 57%)
Gene names: POLI, RAD30B
Sequence domains: impB/mucB/samB family
Structure domains:
DNA (5'-D(*TP*CP*TP*(EFG)P*GP*GP*GP*TP*CP*CP*TP*AP*GP*GP*AP*CP*CP*(DOC))-3') Chains: B, C
Molecule details ›
Chains: B, C
Length: 18 nucleotides
Theoretical weight: 5.53 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

3 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P6522
Unit cell:
a: 97.298Å b: 97.298Å c: 202.906Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.217 0.214 0.261
Expression systems:
  • Escherichia coli
  • Not provided